We narrowed to 566 results for: PAC

1. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   ...trans-splicing, and protein replacement to reverse the impacts of this disease.  As a non-degenerative, monogenic...

2. Viral Production at Addgene

   Type

   Blog Post

   ...cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell culture...

3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   ...decrease. Furthermore, the size of the parts also impacts ligation efficiency, as concentration does not ...

4. CRISPR 101: Any Base Transversion Editors

   Type

   Blog Post

   ...across from the abasic site before filling the empty space. Over several rounds of rational mutagenesis, the...

5. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   ...plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These are transfected into target cells...

6. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   ... of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant...

7. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ...and can include the pressure to publish in high-impact journals, and the requirement for a high grant ...

8. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   ...excluding the PAM sequence. PAM Sequence - The Protospacer Adjacent Motif (PAM) sequence for the nuclease...

9. Mesothelioma - Causes, Symptoms, and Treatment

   Type

   Blog Post

   ...into mainstream use, they will have a much greater impact on the life expectancy and overall survival of ...

10. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    ...mice that may have no mutation, costing money and space. As a rule, screen all of your pups unless you have...

11. COVID-19 Resources

    Type

    Collection

    ...neutralization assays and links to popular envelope and packaging plasmids. Ginkgo Bioworks Plasmid Collection -...

12. Rett Syndrome

    Type

    Collection

    ...trans-splicing, and protein replacement to reverse the impacts of this disease. Addgene is working with the RSRT...

13. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    ...more model organisms that have left a historical impact on the scientific community. (And, check out this...

14. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    ... editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post...

15. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    ...size (~1500 bp) makes iGECInano suitable for AAV packaging.    Find iGECInano plasmids here!   Matlashov ...

16. Tips for Getting a Faculty Position

    Type

    Blog Post

    ...research will advance the field, create a new field, impact disease treatment, etc., again in clear terms. ...

17. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    ...controls only address one type of error that can impact your western blot, and depending too much on normalization...

18. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ...the target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test...

19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    ...into newly synthesized DNA. This labeled ssDNA is packaged into phage and then extracted. Besides taking ...

20. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    ...development of a new technique. In a structure as tightly packed and interwoven as the brain, robust single-cell...