We narrowed to 1,017 results for: PLE;

...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...material pages associated with each item. There are multiple CRISPR tools available as lentivirus and we’ll...ll be adding more as the need arises. For example, we’ve teamed up with the Broad Institute to provide...the publications associated with these libraries. Please note, the pooled libraries we distribute in lentivirus...or are thinking about using our viral services. Please send any questions or comments to help@addgene....

...and therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s ...titers for successful transduction. In another example, neuronal gene expression by an anterograde AAV...visualize expression of fluorescent proteins, for example, as many viral particles must infect a cell to ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...possible, use low-bind or siliconized tubes to store samples as it is more difficult for viral particles to ...

... counter, lab timer, dilution calculator, and a couple of other nice features. This app also has a great...universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with research! It...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information...can safely say that I still have the single and triple abbreviations of the amino acids memorized, but...researchers in the lab. If you have a favorite lab app, please share it in the comments section below! Many thanks...

...efficient product of HMOs that could be used to supplement newborn diets and promote the formation of a ...breaks down the HMO of interest into its component simple sugars (including lactose), and a lac operon controlled...to determine the amount of HMO originally in the sample. The second sensor is a fusion protein consisting...researchers to engineer healthier microbiomes in people all over the world. Toward this end, she recently...training and wants to make her work understandable to people both inside and outside the scientific community...

...more consistent, and more pleasant for everyone, which in turn encourages people to share more, since they...positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than other institutes...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

...equivalent of crossing off days on the calendar after completing your writing goal, which can serve as positive...graph of which days of the week you most often completed your habit is useful for figuring out when to ...makes suggestions on how to make your writing less complex. While I don’t think you should accept all of the...jargon in your writing. While it’s not possible to completely avoid jargon, this tool can help you avoid overusing...tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique...

...make socially distanced work more creative. An example could be making lab meetings more interactive and...and brainstorm! Image from Joseph Mucira.   Implement new tools for creative interaction During Zoom...promote creative interaction. A great tool that a few people use is the built-in whiteboard, in which participants...GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing a multi-authored...journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration. PLoS Comput...

...to the Cas9/tracrRNA complex. D) The mature crRNA is used to guide the Cas9 complex to the target DNA which...functions of the various CRISPR/Cas9 components. Please note that while this post is intended to provide...requirements of these different systems can vary (for example, xCas9 is a variant with increased PAM flexibiliy...remainder of this post, we’ll refer to this RNA complex as the “gRNA”. The gRNA contains both the 20 nucleotide...

...Sharp. After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what...what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been discovered...he says, “In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful... [at the Broad] too… to make science easier for people everywhere…. the nonprofit, sharing ethic that ...comb through the literature, to go and talk to the people who actually have these plasmids, figure out what...

...chronologically. With a resume however, most often people choose a reversed chronological style (the latest...and tailor the resume for the job posting. For example, if the job asks for experience in “statistical...necessary in order for you to do your research. For example, did you help put together a grant application?...be meaningful for your potential employer. For example, my experience in science outreach and writing ... into a communications role instead after she completed her MSc in Experimental Medicine. She is the Community...

...cell imaging experiments is the ability to follow multiple fluorescently tagged species simultaneously. To...do so with fluorescent protein labels requires multiple fluorescent proteins whose excitation and emission... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...

... with your CV to people you’d like to work with next.” Connie Cepko looks for people who are dedicated... and interested in science. “Postdoc hires are simpler because of their documented history in science....satisfaction (especially for scientists and technical people) is linked to engagement and access to growth opportunities...Finding your dream lab While none of us will feel completely happy at work all of the time, the goal is to...

...neuroscience experiments often rely on multiplexing, where multiple fluorescent tools are used simultaneously...can image multiple brain regions or cell types at once using existing indicators. For example, you might...jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently developed a new orange...

...and even allowing multiple proteins to be visualized simultaneously. Complementation systems for examining...For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime... with incompatible substrates can also allow multiplexing with experiments that supply both substrates... Multiple Authors...

...Cis/trans activity, PAM requirement, gRNA, and multiplex ability Experimental guidelines and applications... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ...you need more nitty-gritty details — how can I multiplex this enzyme? What other RNA components are required.... For each enzyme, it will also show you how multiplexed arrays would be designed, if that’s your end ...details to refine your search (trans activity, multiplex ability, gRNA design, etc.). With this feature...

...present in the system at a given point in time. For example, if ATP was the molecule of interest (analyte),...depending on the biology you are studying. For example, calcium dynamics in the cytosol and sarcoplasmic... have been tested in vivo, in tissue preps, in multiple species, and in cell culture models. Some of them... them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...all have specific sensors designed for them. For example, this class includes tension monitors associated...

...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce...administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more synthetic...enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus, or ...applications these viruses are good for, we have plenty of information on that too. May your titer always...

...applications, while electroporation of a human, for example, isn’t a reasonable option. Since viruses used...used in labs are derived from viruses that infect people in nature, extra safety measures, like replication...therapies have already been approved in the US for multiple diseases, including hemophilia B and spinal muscular... immunodeficiency virus type 1 preintegration complexes.” Proc. Natl. Acad. Sci. U.S.A. 89 (1992) 6580... Multiple Authors...

...help my research and my career. Here are three examples of how using social media has helped me or my ... can start using Twitter and provide real-life examples of how they and others have used Twitter professionally...also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what...do you do? — Stephani Page, PhD 👩🏾‍🔬💜 (@ThePurplePage) February 13, 2014 2. "The Scientist's Top... for Twitter pros as well, like tip #4: “Follow people for about a week and then unfollow those who do...

.... This luminescent technology is based on the principle of bioluminescence resonance energy transfer (...photobleaching/phototoxicity, they successfully imaged multiple subcellular compartments over several minutes ...improve long-term imaging, which is limited by depletion of luminescent substrate, they synthesized diacetyl... handy. This approach is especially suited to complement optogenetic tools, where light used for excitation...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern...