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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 761 results for: Rest

Showing: 521 - 540 of 761 results

  1. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...producing a final plasmid that expresses the shRNA of interest. C.1 Recommended Materials Material Vendor and...for plasmids that were successfully ligated by restriction enzyme digestion. However, once you have identified...use a miniprep kit to obtain DNA. 3. Conduct a restriction digest with EcoRI and NcoI: 1 μg miniprep DNA...select for cells that stably express your shRNA of interest. E.1 Recommended Materials Material Vendor and...cells that are stably expressing your shRNA of interest. F.1. Recommended Materials Material Vendor and...

  2. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...DNA from an Agarose Gel You may also like... Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis...technique for molecular cloning, such as PCR - or restriction enzyme -based cloning. Protocol: Gel Purification...lanes between samples and between the ladder and nearest sample. To minimize the risk of DNA damage, it ...

  3. Immunocytochemistry

    Type
    Protocol
    ...depending on the sample type and the protein of interest. You may need to try a variety of fixation methods...transfected with a plasmid to express the protein of interest, and a negative control sample such as cells that... that do not express the protein of interest....

  4. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...dithiothreitol, DTT), Invitrogen NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips...the hot plate to 100 °C. Thaw IgG standard and prestained protein ladder on ice. Reagent Preparation Prepare... that the chamber is sealed. Load 5 µL of the prestained protein ladder to the appropriate well. Pro-Tip...

  5. CRISPR Library Amplification

    Type
    Protocol
    ...high-throughput next generation sequencing (NGS). Select a restriction enzyme for digest that will cut the shared plasmid...visualize that digestion on an agarose gel ( see restriction digest protocol here ). Lentiviral plasmids can...closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing at 30 ℃...

  9. Protocol - How to Design Primers

    Type
    Protocol
    ...complementary regions Note: If you will be including a restriction site at the 5’ end of your primer, note that ...the enzyme to cleave efficiently (e.g. GCGGCG-restriction site-your sequence). Protocol Video Watch the...

  14. Call for Guest Bloggers

    Type
    Blog Post
    ... Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. ...

  17. CRISPR Cheat Sheet

    Type
    Blog Post
    ...resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR Precise edits are difficult...

  18. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...some great costumes this Friday. So if you’re interested in finding out who wins this year’s Addgene costume...

  20. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...#ChristmasScene #womenwhoscience #scigirls #wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication...
Showing: 521 - 540 of 761 results