We narrowed to 570 results for: SHA

1. Viral Production at Addgene

   Type

   Blog Post

   ...references Resources at the Addgene blog Find and share AAV Data with Addgene's Data Hub Digital Droplet...

2. Viral Vectors 101: Preparing Pooled Libraries

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   Blog Post

   ...depositing lab whenever available. Addgene also shares the protocols we’ve developed for the libraries...

3. Tips for Writing a Good Cover Letter

   Type

   Blog Post

   ...briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based: if ...

4. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   ...Jain and Srivastava 2013). They are also easily-shareable tools within the scientific community, as they...

5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   ...and loxP sites differ at the nucleotide level but share an overall structure of 13 base pair palindromic...

6. Changing Labor Laws Bring Increased Postdoc Wages

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   Blog Post

   ...population, and it is unclear how this will all shake out. In the short term, postdocs and potential postdocs...

7. The Challenges of Cell Culture

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   Blog Post

   ...majority of cell line problems come from people sharing cells: the black market of cell biology. Engaging...

8. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...research benefits when the advances of some are shared with all. Due to the versatility and standardization...

9. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...reducing the temperature or moving it to a bench-top shaker 10-20 minutes before adding the inducer. Growth...

10. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post

    .... PubMed Central PMCID: PMC4752446. 5. Asuri, Prashanth, et al. "Directed evolution of adeno-associated...

11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    Blog Post

    ...23287718. PubMed Central PMCID: PMC3795411. 2. Mali, Prashant, et al. "RNA-guided human genome engineering via...

12. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post

    ...more about antibodies  Read about another reagent sharing platform for antibodies, the Developmental Studies...

13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ...Azhar, M., Phutela, R., Ansari, A. H., Sinha, D., Sharma, N., Kumar, M., … Maiti, S. (2020). Rapid, field-deployable...

14. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post

    ...pri-miRNA, which is then processed by a complex called Drosha to pre-miRNA. miRNA plasmids are engineered to ...

15. Hot Plasmids: Summer 2024

    Type

    Blog Post

    ...find a recombinant anti-GFAP [N206A/8R] antibody shared by James Trimmer’s lab (Figure 5) as well as antibodies...

16. Tips for Getting a Faculty Position

    Type

    Blog Post

    ...positions in biomedical science. My motivation was to share my experiences from my own job search and my time...

17. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    ...Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include...

18. Hot Biosensors 2022: Year-End Roundup

    Type

    Blog Post

    ... vivo use and deep-tissue imaging. Vladislav Verkhusha’s lab has generated a new genetically encoded calcium...

19. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

    ...doi.org/10.1371/journal.pone.0154027 Wang Y, Cheng X, Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous...

20. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    ...PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl sulfoxide-mediated primer Tm reduction...