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Viral Vectors 101: The Retroviral Lifecycle

Type

Blog Post

...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...
Summer SciComm Series: Modes of Communication

Type

Blog Post

...kind of content are you sharing? Sometimes your subject matter will drive the kinds of communications you...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...worrisome for me since bioethics is an inherently subjective area. For CRISPR, I focused on the fact that,...
Lentiviral Vector Uses and Overview

Type

Blog Post

...VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells...
CRISPR Antimicrobials

Type

Blog Post

...and potency with phages Neither group observed substantial resistance to the CRISPR antimicrobials, and ...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...nuclease to mediate cleavage rather than the multi-subunit complex employed by class 1 systems. A putative...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...transformation and storage begins. Some samples are submitted as bacterial colonies in petri dishes, but close...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...spontaneously and without additional co-factors, substrates, or enzymatic activity – it only requires the...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to...
Supporting Reproducibility with a Connected ELN

Type

Blog Post

...significantly enhances the ability of others to subsequently reproduce the research. A third, powerful example...
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

... Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution of enrichment...
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

...doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...
Hot Plasmids Spring 2024

Type

Blog Post

...-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii...
Transferable Skills Guide: Creativity

Type

Blog Post

.... It is important to note that the person who submitted the idea did not have to be in charge of implementing...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...antibody that will be used for detection in a subsequent Western blot. When choosing beads, there are ...
Viral Vectors 101: Pseudotyping

Type

Blog Post

...rabies dG enters this neuron, it cannot infect any subsequent neurons because it does not express rabies glycoprotein...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

...resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ...

Showing: 521 - 540 of 577 results

Results per page:

Viral Vectors 101: The Retroviral Lifecycle

Summer SciComm Series: Modes of Communication

SciComm: Taking Science to Elementary and Middle Schools

Lentiviral Vector Uses and Overview

CRISPR Antimicrobials

Tips for Screening with Yeast Two Hybrid Systems

Cpf1: A New Tool for CRISPR Genome Editing

Tips from the Repository Trenches: Using Barcodes to Track Samples

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Supporting Reproducibility with a Connected ELN

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Twenty Years of Sharing: Addgene's Viral Vector Service

Hot Plasmids Spring 2024

Plasmids 101: Broad Host Range Plasmids

Transferable Skills Guide: Creativity

Antibodies 101: Immunoprecipitation

Viral Vectors 101: Pseudotyping

CRISPR-based Genome Editing Systems For Editing in E. coli

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