We narrowed to 581 results for: SUB

1. SciComm: Taking Science to Elementary and Middle Schools

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   Blog Post

   ...worrisome for me since bioethics is an inherently subjective area. For CRISPR, I focused on the fact that,...

2. Isolating Genomic Regions of Interest with the CRISPR System

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   ...genomic locus, which they then confirmed by PCR. Subsequent mass spectroscopy studies allowed for the identification...

3. Lentiviral Vector Uses and Overview

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   ...VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells...

4. CRISPR Antimicrobials

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   ...and potency with phages Neither group observed substantial resistance to the CRISPR antimicrobials, and ...

5. Tips for Screening with Yeast Two Hybrid Systems

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   ...entire proteome of a particular cell type, or a subset based on a cellular compartment or growth stage...

6. Cpf1: A New Tool for CRISPR Genome Editing

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   ...nuclease to mediate cleavage rather than the multi-subunit complex employed by class 1 systems. A putative...

7. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ...transformation and storage begins. Some samples are submitted as bacterial colonies in petri dishes, but close...

8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...spontaneously and without additional co-factors, substrates, or enzymatic activity – it only requires the...

9. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   ...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed...

10. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR...

11. Hot Plasmids - August 2020

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    ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...

12. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ... Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the distribution of enrichment...

13. Transferable Skills Guide: Creativity

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    .... It is important to note that the person who submitted the idea did not have to be in charge of implementing...

14. Antibodies 101: Immunoprecipitation

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    ...antibody that will be used for detection in a subsequent Western blot. When choosing beads, there are ...

15. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...

16. Hot Plasmids Spring 2024

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    ...-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity...

17. Plasmids 101: Broad Host Range Plasmids

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    ...Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii...

18. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...quencher from the proximity of the fluorophore and subsequently leading to a positive signal. The same effect...

19. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to...

20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which...