We narrowed to 731 results for: Sis;

1. Phage Directory: From Phage Therapy to a Repository of Phage Information

   Type

   Blog Post

   ...Twitter to find phages to treat an antibiotic resistant bacterial infection, she shared that tweet with...

2. X-CHIME: Context Dependent Germline Knockout in Immune Cells

   Type

   Blog Post

   ...are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and S-CHIME, we discovered that...

3. Six Spooky Science Stories and Halloween at Addgene

   Type

   Blog Post

   ...encode P450 enzymes that mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular...

4. Science as Art

   Type

   Blog Post

   ...motivated to get data, publish in time, defend the thesis, etc. Photography is critically important in many...

5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

   Type

   Blog Post

   ...require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing...

6. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

   Type

   Blog Post

   ... Be?” In this excellent essay Acclimatrix, an assistant professor at a research university in the northeast...

7. Working 9-5: an Addgene Co-op

   Type

   Blog Post

   ...uses something called “core hours”. Core hours consist of the time between 10 AM and 3 PM, when most meetings...

8. Scientific Sharing in the Time of COVID-19: Databases and Resources

   Type

   Blog Post

   ...researchers in other disciplines (bioinformatics, image analysis, AI…).  How to put the wider scientific community...

9. What's New in CRISPR - March 2020

   Type

   Blog Post

   ...expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional repressor...

10. New Norepinephrine Indicators: nLightG and nLightR

    Type

    Blog Post

    ... Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References and resources...

11. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type

    Blog Post

    ...imaging approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the...

12. Mapping the 4D nucleome with CRISPR/Cas9

    Type

    Blog Post

    ...chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon of useful...

13. Future of Research Conference - Remarkable Opening Session

    Type

    Blog Post

    ... away from PI driven basic research, there is resistance to change, public support is weak, government...

14. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type

    Blog Post

    ...nature, with the ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently...

15. 9 Tips to Achieve Success in Academia

    Type

    Blog Post

    ...aware of careers outside of academia. How do PIs assist their mentees who have goals outside of the university...

16. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type

    Protocol

    ...like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and ...purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the following solutions...phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom phase...

17. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    ...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease....

18. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type

    Blog Post

    ...mechanism called depurination. In depurination, the hydrolysis of the glycosidic bond leads to the release of...

19. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type

    Blog Post

    ...2011) Power tools for gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https:...

20. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    ...configuration. In addition, D10A editing efficiency is consistently higher than that of H840A, especially with smaller...