Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 740 results for: Sis;

Showing: 521 - 540 of 740 results

  6. Let There Be LITE Plasmids

    Type
    Blog Post
    ...ingredients - CRY2 and CIB1 – which they borrowed from Arabidopsis thaliana. The results are light-inducible transcriptional...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and ...purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the following solutions...phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom phase...

  11. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...

  15. Science as Art

    Type
    Blog Post
    ...motivated to get data, publish in time, defend the thesis, etc. Photography is critically important in many...

  18. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...

  20. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian transcriptional repressor...
Showing: 521 - 540 of 740 results