We narrowed to 795 results for: Tec
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TypeBlog Post...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...
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Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...you stay connected. If possible, test out the technology before the conference begins.“There were some... -
25 Work From Home Tips for Scientists
TypeBlog Post... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...measurements such as patch clamp recordings. However, technical difficulties such as small fluorescence changes...QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials in worms with fluorescence changes... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI. Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...limitations. First, FRET signals are often difficult to detect, and thus quantify, due to small fluorescence changes...induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours, a time consistent... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post... to learn more about this killer illumination technique! What is a photosensitizer? First, for a few ...genetically encoded photosensitizer. Nature Biotechnology,24(1), 95-99. doi:10.1038/nbt1175. PubMed PMID... -
Pushing the Limits of DNA Assembly
TypeBlog Post... where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it ...understanding the minimal genome. Many of these techniques require more complex DNA assemblies and improvements... -
Addgene Depositors Get More Citations
TypeBlog Post...plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular...research focuses on a range of topics including biotech and research tools, computing performance, and ... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...contrast to recent genome engineering technologies, stem cell techniques are neither fast nor inexpensive.... -
5 Great Apps for Lab Life
TypeBlog Post...Culture by Gibco Whenever I start to learn a new technique, I try to look for a resource to help me. I’m .... When I started learning proper cell culture technique, I tried to find an app that could help me with... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts Suggest ... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant... -
Fluorescent Proteins 101: Luciferase
TypeBlog Post...indicator of transcription within cells, where detection of the reporter protein or its enzymatic activity...regions on gene expression can be determined by detection of the reporter in a specific assay, which ideally... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection... -
Clear and Accessible Writing
TypeBlog Post...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...also activating or inhibiting gene expression, detecting neuronal signaling, or manipulating any other ...Others have noted a lack of transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe...