...this final installment, I’ll provide a brief introduction as to how you can engineer new transgenic flies...use attP sequence docking sites so that you can control exactly in which locus your transgene ends up (...selection markers include vermillion (another gene controlling eye color) and yellow (a gene affecting the body...

... give you theirs! Think about how you want to introduce yourself and what you do. You can probably use... at a conference, but start with a more general intro until you know if the person you are talking to ...people in sessions by sitting next to someone and introducing yourself. Put your phone away! This is a natural...

.... Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect with complete strangers... much better to use your network to request introductions to people of interest for such discussions.  ...connection can help you gather information, make introductions, and give advice for your interviews.  Pro tip...

... molecule degraders” recruit the proteasome to destroy targeted proteins. We previously developed small...lines Treat parental cells (or cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with...: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia. Nature...

...,000 people globally. Pustules form from the uncontrolled activation of the interleukin-36 (IL-36) cytokine...macrophages, T cells, and the recruitment of neutrophils. Though there are IL-36 receptor inhibitors in... significantly reduced levels in women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene...

...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals...Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also started our blog and YouTube...biology and cloning, since they have reasonable introductions to lots of molecular biology,” — Reddit commentor...

...communicating the results of the work. Yes, those control experiments are important - but without letting...and finish that experiment (and don’t forget the controls!). And when you’re ready to write up the paper...blog Summer SciComm Series: Modes of Communication Intro to Our Summer SciComm Series!...

...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent...concentration 50% of the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization)...respectively, are used as positive and negative controls for proper ER structure. Based on these data, ...

...attached to a substrate. These in vitro methods allow for a well-controlled environment, but they do not mimic...large number of emitted photons before they are destroyed. Factors that affect photostability The photobleaching...

...found that the study saved lives. Reduced use of estrogen plus progestin hormone therapy resulted in 76,...they worry about needing more study subjects and control groups. The important point, however, is that cost...Evoloving Check Out Extracurricular Activities for a Strong Science Career Path Additional Resources Check...

...This operon is under the transcriptional control of a strong constitutive promoter (BBa_J23100) and expresses...blue light.  Similarly, light can also be used to control homo-oligomerization of a protein fused to CRY2...

...including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways...

...find a title, author names and affiliations, introduction, methods, results, conclusions, and acknowledgements...flanked by two equally-sized columns, where the introduction, methods, conclusions, and acknowledgements ...the work. The other poster sections like the introduction, methods, and figures flank the central region...

...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or.... Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand...breaks, a new DNA sequence can be knocked-in, or introduced, into the break sites. Knock-in experiments are...

...MTA) approval  Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... typically includes a couple of sentences of introduction to my area, a line about the research I will...you can find some great information on ELISA troubleshooting and multiplex assays.   Additional Resources...

...sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed gene circuits ... an extensive infrastructure of molecular and electronic companion technologies that has transformed the...CRISPR/Cas9-mediated genotyping, as well as our electronic reader that provides portable quantification ...

... recombinase.   Once the SSR of choice is introduced, recombination can proceed either by first utilizing...alone rescues the phenotype being studied. Introduction of a conditional point mutation or functional...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...

...background without an introduction. If someone approaches your poster, introduce yourself and ask about...have extra time to practice at the actual venue. Introduce yourself: I always find it odd when I walk up ...

...pitch,” the 30 - 60 second blurb you will use to introduce yourself and what you do. You might want to include...research. Try to avoid jargon in this initial introduction. During the conference When you meet someone...is what their project is. Or maybe you want to introduce yourself to a scientist whose work you are familiar...