Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 750 results for: Uty

Showing: 521 - 540 of 750 results

  4. Reflections and Looking Ahead

    Type
    Blog Post
    ...thoroughly characterized antibodies, and our contributions to key research papers are already inspiring...

  6. Editor's Choice, August 2016

    Type
    Blog Post
    ...how to give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend taking...

  10. AAV ddPCR Titration

    Type
    Protocol
    ...in the dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer ... carefully add the dilution buffer to the dilution plate according to the dilution scheme listed in step...Pro-Tip Prepare the viral dilutions in a separate biological safety cabinet (dilution BSC) than that used for...touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well freezer... add 5 µL of each viral sample to Dilution 1 in the 48-well dilution plate and pipette 5–10 times to mix...change tips each time. Dilution 1 (20X): 5 µL in 95 µL 1X PCR buffer (1:20) Dilution 2 (20X): 5 µL in 95 ...PCR buffer (1:400) Dilution 3 (20X): 5 µL in 95 µL 1X PCR buffer (1:8,000) Dilution 4 (20X): 5 µL in 95...

  15. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...of 2 x 10 8 dilution 90 2 x 10 7 10 of 2 x 10 7 dilution 90 2 x 10 6 10 of 2 x 10 6 dilution 90 2 x 10 ...Total dilution Dilution 1 (DNase step) 5 uL AAV stock 45 uL 10X 10X Dilution 2 5 uL Dil. 1 95 uL 20X 200X...200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution 4 20 uL Dil. 3 80 uL 5X 5000X Dilution 5 20 uL Dil...25000X Dilution 6 20 uL Dil. 5 80 uL 5X 125000X Dilution 7 20 uL Dil. 6 80 uL 5X 625000X Dilution 8 20 ... To obtain a solution at 2 x 10 9 molecules/μL: 1.59 x 10 11 / 2 x 10 9 = 79.8X dilution ...10 5 10 of 2 x 10 5 dilution 90 2 x 10 4 10 of 2 x 10 4 dilution 90 2 x 10 3 Pro-Tip To help stabilize ... standard dilution. Dilute DNase-treated and AAV reference samples according to the dilution scheme in...
Showing: 521 - 540 of 750 results