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  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies widely with gRNA sequence. Both groups used multiple...Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1...

  3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...within each color also exist with only slight variations separating them.  Please note that many fluorescent...fluorescent biosensors has been designed to detect a variety of intracellular conditions, including ion (such...

  4. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...collection of lentiviral plasmids created for a variety of applications including cDNA expression, shRNA-mediated...breaks down the biosafety concerns associated with various aspects of lentiviral transduction along with suggestions...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...indeed.com or monster.com, where you’ll find a variety of listings. Post your CV and make it visible, ...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ... able to quickly synthesize information from a variety of fields in order to provide useful solutions ...been a long brewing frustration for me. I read a variety of psychology, business, personnel, and sociology...

  7. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ... participate in forum discussions about a wide variety of topics. Individual forums are hosted on subreddits...sometimes, civil. r/Science in particular hosts a variety of conversations with people who work in many different...

  8. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...3645–3650 https://doi.org/10.1073/pnas.0909910107 Varani G, McClain WH (2000) The G·U wobble base pair. ...

  9. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post
    ...Since the efficiency of CRISPR/Cas9 genome editing varies across targets, they tested two genes in mice. ...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  11. CRISPR-mediated Plant Base Editors

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    Blog Post
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...

  13. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...save researchers significant time and removes a variable from the lengthy process of validating and using... (Figure 4), our catalog includes vectors in a variety of serotypes, including the retrograde AAVrg and...

  14. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...way for the cell to control DNA replication. A variety of other proteins also contribute to this regulation...emboj/17.17.5201 del Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998...

  15. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...domain conferred several new improvements. The new variant has increased signal-to-noise ratio and better ...disease-relevant large proteins. They demonstrated REVeRT in various contexts in vitro and in vivo, including in human...

  16. CRISPR Activation: A Practical Guide

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    Blog Post
    ... Cas9 activator should I use? There are a wide variety of activators you can use for your experiments....Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next Experiment Find out More about Genome-wide...

  17. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ...expression? Low expression outcomes can result from variety of sources, so fear not—there are a few simple ...up a small-scale expression experiment to test variables such as temperature, time, and media conditions...

  18. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...genome editing. In this post we'll walk through a variety of ways you can use AAVs to improve your genome...being the vector of choice for gene delivery to a variety of cell types, now you know that AAVs can provide...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...homology arm, but keep in mind these numbers may vary based on your locus of interest and experimental...ssODNs in mouse models. Although HDR efficiency varies across loci and experimental systems, ssODN templates...
Showing: 521 - 540 of 767 results