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Showing: 521 - 540 of 1090 results
  1. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...019-46171-2. Resources on Addgene.org Modular Cloning (MoClo) Guide Addgene’s eBook Collection More resources... will review common uses of MoClo and the kits Addgene offers to kickstart these applications, no matter...starter for your general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant...CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too. The AspFlex... (Brychcy et al., 2023). For plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits...study in yeast cells. Need more MoClo? Check out Addgene's MoClo Guide! Customizing MoClo for your needs ... resources on the Addgene blog Plasmids 101: Golden Gate Cloning High Complexity Golden Gate Cloning ...
  2. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post
    ... an ambitious goal of curing Rett Syndrome and Addgene is thrilled to partner with them to launch a Rett...passion for facilitating research and discovery, Addgene and RSRT have consolidated and centralized a collection...Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith Joung, PhD, and...the root cause of Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will...deposit their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to...
  3. Filming Science Videos in the Age of Social Distancing

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    Blog Post
    ...Are the shots clear enough for the audience? As Addgene’s Science Video Production Specialist, I’m constantly...March 2020, just four months after I started with Addgene, I was about to have a check-in with my boss Jennifer...traditional solution was completely out of reach for Addgene.  So, onto research! I needed to design a system...the Lab Design: The lab is the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing... out all of the written and video protocols at Addgene? If you need help in the lab, we have you covered...some incredibly strict safety protocols to keep Addgenies safe. Space was at a premium, so I had to figure...
  4. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ...If you’re been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you...placing the aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the... we’ll take a deeper dive into the content of Addgene’s aptamer soup by exploring the development and ...and applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers Spinach...PMCID: PMC4143336. Additional resources on the Addgene blog Learn more about aptamer fluorophores Investigate...Find other uses for RNA aptamers Resources at Addgene.org Browse fluorescent protein plasmids and resources...
  5. Hot Plasmids Spring 2024

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    Blog Post
    ...sequencing, and optical pooled screens in human cells. Addgene offers vectors for CROPseq-multi using selection...recombinant antibodies were recently added to the Addgene repository of tools for SAR-CoV-2 research. Two...critical component for viral genome packaging. Addgene recommends both for use in western blots (Figure...as a loading control (right panel). Image from Addgene. These antibodies are recombinant versions ... out the new and noteworthy tools featured in Addgene’s Neurodegeneration Research Collection. This page...
  6. Antibodies 101: Fab Fragments

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    Blog Post
    ...Mess in IHC Resources on Addgene.org  Browse ready-to-use antibodies at Addgene...visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized...when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken up, there...j.crmeth.2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment...
  7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
    ...References on Addgene.org Check out Addgene's Chemogenetics Guide Find fluorescent proteins at Addgene ...fusions, check out Joachim Goedhart’s article on the Addgene blog: Fluorescent Proteins 101: GFP Fusion Proteins...0471140864.ps0909s73 Additional references on the Addgene blog Browse Fluorescent 101 blog posts Read about...
  8. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...Services  Resources on the Addgene blog  Viral Vectors 101: Biosensors Viral Vectors 101: Viral Applications... to AAV Find AAV Calcium Biosensor Vectors at Addgene ...
  9. Viral Vectors 101: Virus Safety

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    Blog Post
    ...Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide Addgene Viral Plasmids ... Type of Virus Should I Use? Resources on the Addgene blog Viral Vectors 101: Types of Viruses Viral...
  10. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...More Resources at Addgene:  Check out more Plasmids 101 Posts! Check out Addgene’s restriction cloning...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...can also be adapted to express multiple gRNAs. Addgene has two Golden Gate-based gRNA assembly methods...
  11. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    Blog Post
    ...manipulation Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors...tools for optogenetics and chemogenetics on the Addgene website. Optogenetics also requires that light ...bs.mie.2018.01.022 Additional resources on the Addgene blog Read more about optogenetics New to viral...
  12. What's New in CRISPR - March 2020

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    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...
  13. Reflections and Looking Ahead

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    Blog Post
    ...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...
  14. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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    Blog Post
    ...Children’s Hospital and Harvard Medical School. Addgene is proud to present a video reprint of the CRISPR...the full protocol from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank...Browse CRISPR Plasmids CRISPR Information at Addgene More CRISPR-Cas Blog Posts    ...
  15. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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    Blog Post
    ...and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR... to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents out ...  New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale...
  16. Congrats to the 2019 Deck The Lab Winners

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    Blog Post
    ...EikeMahlandt @AnoeskaMoosdijk @Saskia_deMan @SILS_UvA @addgene #SciArt #fluorescence #LCAM #DeckTheLab pic.twitter.com...holiday season. Santa frog is coming to town. @Addgene @UMich_MCDB #christmastaco #frogsnowflake #xenopus...
  17. Twenty Years of Sharing Science!

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    Blog Post
    ...Today marks Addgene's 20th anniversary! We've done so much in the past two (2!) decades that it's hard...publication by pasting the paper's title into the Addgene website search bar?   We'll be celebrating all ...protocols, and of course, the friendly folks at help@addgene.org! Fun fact! Did you know you can search for plasmids...
  18. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post
    ...resources on Addgene.org IPI Antibody Collection Antibody Guide Addgene Antibody Collection Addgene Antibody...especially when sharing with other scientists. Addgene partners with IPI to provide recombinant antibodies...data from IPI and other labs easily viewed in Addgene’s Data Hub. The diversity of antibody reagents is...button_text" is_json="true" %}{% raw %}"Visit Addgene's Data Hub"{% endraw %}{% end_module_attribute %...0471142727.mb0803s51 Additional resources on the Addgene blog All Antibody Topics Antibody Data Repositories..."href":"https://datahub.addgene.org/","href_with_scheme":"https://datahub.addgene.org/","type":"EXTERNAL...
  19. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post
    ... of the vector you are using. Contact Addgene (help@addgene.org), or the supplier of your vector, to obtain...Technologies Resources on Addgene.org Find ready to use AAV vectors at Addgene. Find AAV plasmids to use...one of the most common concerns we get here at Addgene about this system is off-target or spurious expression... cells that do not express Cre or Flp. Here at Addgene we quantify the amount of transgene recombination...2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination rate (0.1%.../fnmol.2020.618020 Additional resources on the Addgene blog Plasmids 101: FLEx Vectors Important Considerations...
  20. Finding Your Perfect Job After University

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    Blog Post
    ...perfect job.   Resources at Addgene Read Additional Career Posts on the Addgene Blog Browse Our Careers ...applied anyway. I later found out the job was with Addgene. I had used plasmids from this company before and...also get the chance to contribute content to the Addgene’s Website and travel to meet scientists, making ...develop my own projects, but working remotely (Addgene’s main offices are in Cambridge MA, while I’m based...Careers Page to See What Positions Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional...
Showing: 521 - 540 of 1090 results