We narrowed to 599 results for: arl

1. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   ...reagents, making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate...

2. Great Results Start with Great Standard Curves

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   ...consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use duplicates or...

3. Antibodies 101: Producing Recombinant Antibodies

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   ..., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor...

4. Getting to Know Expansion Microscopy

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   ...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...

5. Droplet Digital PCR for AAV Quantitation

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   ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...

6. Plasmids 101: Colony PCR

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   ...  PCR set-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction:...

7. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus Conzelmann. "Generation of bovine respiratory...

8. Cpf1: A New Tool for CRISPR Genome Editing

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   .... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which...

9. A Career in Grants: How To Become a Grant Professional

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   ...particular area, e.g. education, poverty, environment, early childhood development, or community development?...

10. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...able to work in different parts of the world in my early research career. I've found that research institutes...

11. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ... type of transcription reporting was among the earliest uses of GFP. Fluorescence resonance energy transfer...

12. Negotiating Work and Life: How to Find the Joy

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    ...you’ll never know it so don’t miss out on those early kisses. The laundry or dishes can wait. Also, be...

13. Plasmids 101: Codon usage bias

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    ...Central PMCID: PMC1299251. 3. Emilsson, Valur, and Charles G. Kurland. "Growth rate dependence of transfer...

14. Neuronal labeling with Spaghetti Monster

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    ...reliable primary antibodies for their detection, particularly when antibodies to the POI are non-specific,...

15. CRISPR-mediated Plant Base Editors

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    ... other proteins. You can detect base editing as early as 48-72 hours post-transformation/transfection ...

16. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...they saw indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice...

17. Transferable Skills Guide: Creativity

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    ... of encouraging dissent, the next tip may be particularly relevant to you. Elect a devil’s advocate. This...

18. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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    ...systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...depends directly on the choices you make during the early stages of the process. Some questions to start with...

20. Plasmids 101: Shuttle Vectors

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    ...machinery. Alternatively, some slow-growing cells, particularly mammalian cells like neurons, may not replicate...