We narrowed to 643 results for: ato

1. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma proteins ...

2. Plasmids 101: Protein Expression

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   ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...

3. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   ...editing Hu et al. also engineered transcriptional activators using the xCas9 scaffold. With an NGG PAM, xCas9...

4. Transferable Skills Guide: Managing a Team

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   ...delegate - as I quickly learned! I was a poor delegator at first. I knew what needed to be done and how...

5. The Scientific Conference Poster Session: Tips for Success

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   ...minutes per poster. Ask the presenter to give the “elevator pitch,” of his/her project – kindly let the presenter...

6. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...Tsuboi et al.), limiting its use. Nonetheless, its creators noticed a few properties that have now become ...

7. Viral Vectors 101: Producing Your rAAV

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   ...Blouin-Tavel, V., Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R.,...

8. The AAVantages of AAV in CRISPR Screens

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   ...vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831 Santinha...

9. Production of Virus in Insect Versus Mammalian Cells

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   ...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...

10. Magnetic Control of Proteins: More than a Dream

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    ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...

11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a...

12. Academic vs. Industry Postdocs

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    ...time working on something extremely risky or exploratory. Getting a job afterwards: Fairly or unfairly...

13. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ...tool for gene expression in mammals Use the PEI calculator for planning AAV packaging transfections Find...

14. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...catalytically dead dCas9 can be used in conjunction with activator or repressor domains to control gene expression...

15. Tips for Using BLAST to Verify Plasmids

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    ...such as full plasmid sequences provided by the laboratories that deposit their plasmids with us or other...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...world using words like OR and AND. These logical operators are so useful they are implemented in most materials...

17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...technologies. Message or follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg...

18. CRISPR 101: Making a Knock-In Cell Line

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    ... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...

19. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    ... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological...

20. A Guide to Starting Your Own Journal Club

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    ...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal...