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We narrowed to 986 results for: des

Showing: 521 - 540 of 986 results

  1. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...inversions, deletions, and translocations. It also provides researchers with many different ways to control...the right one. In this post, Addgenie Melina Fan guides us through the basics of the validation process...experiment? In this post, Addgenie Chari Cortez guides us through some common controls and why you might...

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ... the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for C....America. To use this SEC-based system, first design and clone 500-700 bp homology arms for your gene...injected 60-80 animals per gene, they obtained the desired insertions on the first attempt for 6/7 genes. ...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...As with other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological atypicalities... Joanne Kamens, Executive Director  I Contain Multitudes by Ed Yong "Microbes are everywhere and this ...shocking at times."     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd...

  4. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...distribution of cell lines from repositories on opposite sides of the world shows that science is truly a global... et al., 2006), the hACE2 transgenic mice were designed after it was discovered that SARS-CoV, which entered...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the ...

  5. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction... viral proteins except for Nsp3 and Nsp16, and includes an additional catalytically dead version of the...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, ...

  6. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...abstracts were driven by the quality of the study design or by their preconceived ideas about the controversial...transmission and how different fabrics and mask designs could work to slow the spread of illness. I later...Series: Masters of SciCommSummer Scicomm Series: Modes of CommunicationCareers in Science Communication...

  7. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...phrase gram negative or positive being used to describe a species of bacteria, but what does it actually...bacteria. In a gram negative bacteria, the alcohol destroys the outer lipid membrane, and the thin inner peptidoglycan... is also useful in a laboratory setting. When designing a plasmid to be inserted into a specific bacterium...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...transcription factor library The human genome encodes approximately 1,800 TFs, with >3,500 unique isoforms... and screening mechanisms, although the unique barcodes for each TF help streamline identification. While...a disease in a model system. TF screens can be designed to identify custom TF programs to acutely initiate...

  9. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein...brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will work better with shorter...

  10. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... craft sentences for abstracts and presentation slides, learn from audiences as they do science outreach...about whether the same principle applies to other slides in your deck.  If someone asks a question about...of a new science communication initiative that provides yet more scientists with opportunities to practice...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...engineering approaches and scientific know-how to design/build/test their own project ideas using synthetic...newsletters, pairs teams with STEM mentors, and provides a nationally recognized platform in March to exhibit...s BioBuilderClub teams’ biological engineering designs and ideas ranged from a biological breathalyzer...

  12. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...positive effect on cancer treatment (Ref 1). As described in the presentation, starvation or the use of ...field of Immuno-Oncology, a sizzling hot topic, includes studies on novel cancer therapeutics such as  ... with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel...

  13. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...tables with fluorescent protein properties and provides a quick way to compare fluorescent proteins, e.g...been used in yeast by Lee et al (2013) and in nematodes by El Mouridi et al (2017) and Heppert et al (...practical brightness. Therefore, practical brightness provides a better picture of what can be expected in a ...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...sequence NGG TTTV PAM site preserved? Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing....  Similar to a jigsaw puzzle, the oligos were designed with sticky ends that only anneal together in ...

  15. Antibodies 101: ChIP

    Type
    Blog Post
    ...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works...up the DNA, or enzymatic digestion. Sonication provides a truly random digestion, but is harsher and requires...both tissue or cell type, amount of protein, and desired fragment length for your output assay. A longer...

  16. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ... also easy to run things in parallel. Addgene: Besides being fast and efficient, what other challenges...wanted something that would allow automation. This design cuts out weeks of waiting and hands on labor. That...Addgene, now these can be available to anyone for a modest cost without having to start all over or write ...

  17. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...bacterial lac operon contains a gene called lacZ that encodes for the enzyme β-galactosidase. Expression of the...process is called α-complementation. The system described above was put to practical use in the following...Although blue-white screening is probably the most widespread way to select for plasmids containing an insert...

  18. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...and does not consider the missing or damaged nucleotides that are common to biological sources of DSBs...better substrates for ligation. Although we do not describe these steps here, the processing of DNA ends tends...of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs or...

  20. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ... In frogs, ectopic expression of ion channels, despite having an otherwise normal genomic background, ...Subsequent cutting results in a two-headed worm, despite the fact that it’s genetically wild type  (Oveido...fluorescent voltage indicator with near-complete desensitization in continuous light. ArcLight Q239 - A GEVI...
Showing: 521 - 540 of 986 results