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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16 results for: des

Showing: 1 - 16 of 16 results

  1. Science Guides

    Type
    Guide
    ...Collection of guides that describe how popular scientific techniques work... Science Guides Addgene has created... created the following guides to help you start learning about various scientific concepts related to ...to our plasmid collection. Find links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics... sets. Read More Viral Vectors Find educational guides to learn more about the different types of viruses...

  2. Addgene's eBook Collection

    Type
    Guide
    ...First Edition, trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close About... tips for planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide...November 2018 | First Edition About The guide includes information on the many career options in science...

  3. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Retroviral Vector Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin, S. S...PMID: 24501411 Gilroy, K. L., Terry, A., Naseer, A., De Ridder, J., Allahyar, A., Wang, W., Carpenter, E.... Guides Gamma-Retroviral Vector Guide Gamma-Retroviral ...been widely used in the research community for decades. Wildtype gamma-retroviruses have been engineered...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...lentiviral packaging methods . The three plasmids described above (envelope, packaging, and transfer) are ...into the HEK293T packaging cell line. This system provides the greatest flexibility to pseudotype gamma-retrovirus...

  4. CRISPR Guide

    Type
    Guide
    ...M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries, R., Brouns, S. J., & Van Der...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including...Han, Y., Ogrodowski, P., Crippa, A., Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014)...., Boix, C., Perrouault, L., Tesson, L., Geny, S., De Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli... the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit..., the longer pegRNA also encodes a primer binding site (PBS) and the desired edits on a RT template. The...

  5. Plan Your Experiment

    Type
    Guide
    ...multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location...on proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...protocol. Read more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once your target sequences...selected, it’s time to design your gRNA oligos and clone them into your desired vector. In many cases,...experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment...

  6. Molecular Biology Reference

    Type
    Guide
    ...helix. The order of these 4 nucleotides makes up the genetic code and provides the instructions to make ...thymine in RNA molecules. Every 3 nucleotides (codons) in a DNA sequence encodes for an amino acid. The genetic... transcribed from DNA. DNA is comprised of 4 nucleotides or bases, adenine, thymine, cytosine, and guanine...amino acids. Each amino acid is encoded for by 3 nucleotides termed a codon. As there are only 20 natural ...essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance...some common plasmid elements: Plasmid Element Description Origin of Replication ( ori ) DNA sequence which... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without...

  7. Chemogenetics Guide

    Type
    Guide
    ...a new window) Guettier JM, Gautam D, Scarselli M, de Azua IR, Li JH, et al. (2009). A chemical-genetic...less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), like RASSLs...Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chem Neurosci...Wess J (2012). Design and functional characterization of a novel, arrestin-biased designer G protein-coupled... Science Guides Chemogenetics Guide Chemogenetics Guide Jump To... alternative DREADD ligands. Compound 21, Deschloroclozapine (DCZ), Perlapine, and Olanzapine have all...bidirectional control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons DREADD...

  8. Antibody Guide

    Type
    Guide
    ... is described in more detail in the Multiplex Assays section below. The following table describes the ... Science Guides Antibody Guide Antibody Guide View Addgene's Antibody...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... used to quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate ...isolate specific proteins or protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation...Visualization - used to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry ...

  9. Lentiviral Vector Guide

    Type
    Guide
    ... Interchangeable; usually encodes for VSV-G Interchangeable; usually encodes for VSV-G Safety Safe; replication...integration of the barcodes, which can then be inherited by daughter cells. Inheritance of the barcodes makes lentiviral... Guides Lentiviral Vector Guide Lentiviral Vector Guide...been widely used in the research community for decades. Wild-type lentiviruses have been engineered into...ONLY by a second-generation packaging system that includes the tat gene Can be packaged by both second- and...and eliminates the requirement for Tat. Always includes deletion in the 3' LTR for self-inactivation LTR...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...

  10. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Guides AAV Vector Guide Adeno-Associated Viral (AAV) Vector...inverted terminal repeats (ITRs). The Rep region encodes four non-structural replication proteins (Rep78...replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins...cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...new window) Kwon, I., & Schaffer, D. V. (2008). Designer gene delivery vectors: molecular engineering and...., Schaffer, D. V., & Karpova, A. Y. (2016). A designer AAV variant permits efficient retrograde access...

  11. Cloning

    Type
    Guide
    ...for it to then be digested and ligated into your desired plasmid. It is important to note that restriction..., that cut your insert or backbone at only the desired location for your cloning project. Restriction ...rapidly shuttled into any compatible Gateway® Destination vector, which contain attR sites via LR clonase...clonase enzymes. Thousands of Gateway® destination plasmids have been made with different promoters, tags...polymerases synthesize DNA molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together...stranded DNA fragments can be used, so if properly designed, any insert fragment (PCR product or synthesized... backbone independent of how it was originally designed to be cloned into. A drawback of this system is...

  12. Optogenetics Guide

    Type
    Guide
    ... Science Guides Optogenetics Guide Optogenetics Guide Jump To: ...cell membrane. Microbial opsins, such as those described below, can be targeted and expressed in specific... Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm) Channelrhodopsins:....1 540 Plan Your Optogenetics Experiment When designing your optogenetics experiment, you’ll need to pick...key in optogenetic experiments. Your experimental design will determine whether you’ll need short or long... protein. This principle has also permitted the design of synthetic two-part transcription factors - Cry2...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity...

  13. Adenovirus Guide

    Type
    Guide
    ... Guides Adenoviral Vector Guide Adenoviral Vector Guide...most widely used viral vector platform for vaccine design against a diversity of viruses. This guide contains...with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen plasmid...be harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes...but has, however, proven to be very useful for designing and producing rAdV-based vaccines. Adenoviral ...altering gene activity. Oncolytic Referring to the destruction of cancer cells, generally by lysis. pAdEasy-...

  14. Promoters

    Type
    Guide
    ...gene. The coding strand is the DNA strand that encodes codons and whose sequence corresponds to the mRNA...proximal promoter, and distal promoter. Below describes the specifics of these regions in eukaryotic cells...Pribnow box which usually consists of the six nucleotides, TATAAT. Proximal Promoter Further upstream from...Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from...Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA polymerase...

  15. Guide to Using Pooled Libraries

    Type
    Guide
    ... Science Guides Guide to Plasmid Pooled Libraries Plasmid Pooled...effects of expressing multiple genes at once. A well-designed screen can help you begin to understand what genes...important to a certain phenotype, and allow you to design additional hypothesis-directed experiments. As ...tube. Pooled libraries can be small, if they are designed to cover only a subset of genes, or very large...

  16. Educational Resources

    Type
    Guide
    ...educational resources, including eBooks, science guides, videos, and protocols...Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth instructional content on subjects...
Showing: 1 - 16 of 16 results