We narrowed to 625 results for: emb

1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

2. Addgene: The First Twenty Years

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   ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

3. Viral Vectors 101: An Introduction to AAV

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   ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

4. The time and cost required to make a plasmid

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   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

5. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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   ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

7. The Future of Research Symposium Boston 2015

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   ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

8. New Tool for Lineage Tracing: The ClonTracer Library

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   ...library allows a researcher to label individual members of a starting population of cells with specifc,...

9. Engaging with science and society at pgEd

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   ...’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ...

10. Overcoming the Challenges of Lentiviral Production

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    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

11. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

12. Adenoviral Vector Production and Troubleshooting

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    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

13. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

14. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

15. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

16. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

17. Corralling and Cornering the Goldilocks Antibody

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    ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

18. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

19. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

20. CRISPR in the Clinic

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    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...