Antibodies 101: Affinity Reagents
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...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...
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Transferable Skills Guide: Problem Solving
TypeBlog Post...both in and out of the lab. The next step is to remember that you have these skills when you start applying... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged... -
Addgene: The First Twenty Years
TypeBlog Post...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome... -
The time and cost required to make a plasmid
TypeBlog Post... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These... -
CRISPRainbow and Genome Visualization
TypeBlog Post...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants... -
The Future of Research Symposium Boston 2015
TypeBlog Post...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...library allows a researcher to label individual members of a starting population of cells with specifc,... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the... -
CRISPR in the Clinic
TypeBlog Post... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy... -
Viral Vectors 101: Virus Safety
TypeBlog Post...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...