We narrowed to 626 results for: emb

1. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

2. Addgene: The First Twenty Years

   Type

   Blog Post

   ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

3. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   ...expression for several years (Wonjo et al., 2013). Remember that AAVs do not integrate into the host genome...

4. The time and cost required to make a plasmid

   Type

   Blog Post

   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

5. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

6. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

   Type

   Blog Post

   ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

7. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

8. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   ...library allows a researcher to label individual members of a starting population of cells with specifc,...

9. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

11. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    ...dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...

12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...s research is the mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes...

13. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ...packaging signal (Ψ), both of which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated...

14. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...

15. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    ...technique that briefly destabilizes the cell’s outer membrane and allows the components to physically enter ...

16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type

    Blog Post

    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

17. CRISPR in the Clinic

    Type

    Blog Post

    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

18. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

19. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

20. 6 Tips for Grant Writing

    Type

    Blog Post

    ...about what reviewers in your area are looking for. Remember though, you need to leave them time to read it...