Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 993 results for: lars;

Showing: 521 - 540 of 993 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ... the inability of an organism to synthesize a particular organic compound required for its growth. Many...

  2. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...This system also allows precise modulating of a particular arm of the signaling pathway (i.e. everything...

  3. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 is synthesized...active enzyme. Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy...

  4. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...setup we can observe the whole population of one particular protein in neurons or in any other cell type....attended a scientific symposium called Horizons in Molecular Biology. This annual symposium is organized by...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...interests, suspected to interact, are added to a cellular sample. Both antibodies have a short antibody ...

  5. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...Determining which features to verify will depend on the particular plasmid you are working with, but, in most cases... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...forward and reverse primers). The abundance of a particular barcode sequence then corresponds to the abundance...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

  7. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...of fragments, or the ligation of very small/very large fragments, these problems can be overcome by screening...method, but can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with...leveraged the power of Golden Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo...101: Multiplex Expression of gRNAs Choosing a Molecular Cloning Technique  ...

  8. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein antigen...Boguszewska et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also... visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...and resources page Browse our molecular biology protocols Read our molecular biology reference ...scientists to publish findings that don't fit into larger publications and would otherwise be lost in lab...worm so check back frequently for what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN...

  10. Hot Plasmids February 2024

    Type
    Blog Post
    ...edit due to toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...Ando, R., et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature...with neuron bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly...

  11. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, ...Viruses have become a regular part of basic biological research as well as clinical therapy. These biological...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

  12. Engaging with science and society at pgEd

    Type
    Blog Post
    ...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...bench scientist completing my graduate studies in molecular biology and epigenetics. So, I sought out a number...be understood by those who are not experts in particular scientific fields. As scientists, many of us ...Find all of our science communication posts Extracurricular activities for a strong science career path...

  13. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells...increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system belongs to this category. 3rd Gen...plasmid (~1 week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs...ultracentrifugation to separate rAdV from other cellular debris. Purified high-titer rAdV is then dialyzed...

  14. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...skills Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association...provides a peak into their day-to-day business with popular work experiences of 3-10 days. And the Society ... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

  15. Design Tips for Prime Editing

    Type
    Blog Post
    ...need to design and optimize a pegRNA for your particular edit. These tips are a great starting point: ...also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22...

  16. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...avenues for therapeutics that treat disease at the molecular level. For instance, if a disease is caused by...vectors derived from AAV have recently gained popularity as they are uniquely suited gene delivery vehicles...hemophilia B, LPL deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable...on gene therapy for viral infections. She is particularly passionate about HIV cure research and science...

  17. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... several reasons. Casɸ is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  18. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... your interview The STAR interview method is a popular way to answer behavioral interview questions. STAR...key decision. Even deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

  19. Transferable Skills: Negotiation

    Type
    Blog Post
    ... applied for jobs, I knew I should negotiate my salary after receiving an offer but even as I did (successfully... wearing business suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The...prioritize in a lab with both electrophysiologists and molecular biologists? What is their perception of me and...working with you will benefit them too. Ed's note: Salary negotiations will be covered separately in a future...

  20. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...isolated, and disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers...existing bacterial CRISPRi methods. First, its modular design allows for customization with strain-specific...generations. Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with...be used in non-model bacteria species where its modular nature allows for the customization of guides, ...
Showing: 521 - 540 of 993 results