We narrowed to 810 results for: mal

... SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian cell expression. These plasmids will be useful...expresses all the components of the biosensor in optimal proportions from a vector backbone that allows ...

...dose (TCID50) (Zen et al., 2004).  To choose the optimal titering assay, you’ll need to understand how different...Levites, Y., … Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective...

...five amino acids, meaning the tag could be quite small. However, substance P has an amide group added to...larger than the substance P peptide, but still quite small. However, unlike the substance P tag, they found...

...improved protocol. Many scientists, publishers, and small start-ups are turning to software apps to solve ...you painstakingly complete your experiment – one small victory in a long road to your scientific breakthrough...

...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI ...pWPI IRES Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral...

...to screen for small-molecule inhibitors of a particular PPI. In such experiments, small-molecule inhibitors...

...parties, and the company at large, by polling and informal communications we made a decision to try out a...the other hand if the journal club occurs with a small technical team (single lab or team), a deep technical...

...properly, it is unstable and may only work for a small number of qPCR runs before a new one is needed. ...and the droplet generation oil are moved through small channels to create a water-in-oil emulsion containing...

...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C....

...amount and type of information you want to convey. Small tidbits and fast/fun facts are easy and engaging... be oversaturated. Transition from academic to informal writing can difficult.  Photography Medium...

...answer to the space problem. Instead of imaging smaller, what if we just made the sample bigger? In ExM...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...

...can be used. The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...

...higher Tm give more efficient recombination. We normally use a Tm between 47-55 °C for the homologous region...be very helpful to confirm primer design.  Use minimal template DNA Use 1 ng of PCR template DNA to minimize...

...targeted disease therapies with minimal side-effects on healthy cells and maximal effect on unhealthy cells....

...antimicrobial must reach essentially every cell or else small pools of antibiotic resistant cells could repopulate... systems used in both papers are analogous to mammalian viral vectors in that the vectors are replication-deficient...

...if each gene were encoded in separate (and thus smaller) plasmids. Finally, having four separate plasmids...titration experiments might be helpful to achieve optimal results. It is important to note that this system...

...Magnetic beads are solid spheres and tend to be much smaller than agarose beads. Proteins conjugate on the surface...following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions...

... are also great at infecting neurons and cause minimal inflammatory response, but are not taken up by ...References: Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy...

...entirely. The other advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding...toxic for the cells and affects the labeling of small structures like neurites. Like epitope tags, low...

... the body, where it becomes embedded due to the small, hook-like shape of asbestos fibers. Over time these... HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the cell dies from...