We narrowed to 779 results for: mos
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TypeBlog Post... very useful for genetic engineering approaches. Most importantly for this technology, however, is that...over transfecting with plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike...
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Antibodies 101: Validation
TypeBlog Post...expression in the retina and in parts of the brain, but almost none in the liver (A). In general, RNA levels tend...analyze the resulting data, so it may not be the most accessible option for many laboratories. Additionally... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...Using a DNA template for the RNAi trigger is the most prevalent method due to ease of use and capacity...actively silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly ... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post... is a powerful experimental strategy, one of the most common concerns we get here at Addgene about this...-homologous pairs) (Figure 2.). They found that almost all off-target expression can be attributed to ... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...recognize the same epitope of the target protein. Most commonly these antibodies are produced from hybridomas...fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements. Biosensors... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned into the Cas9-containing destination...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ... -
15 Hot Plasmids from 2017
TypeBlog Post...many applications in biotech, and is considered the most studied Gram-positive bacterium, many of its genes...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops... -
Beginner's Guide to Viral Vectors
TypeBlog Post..., Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog Post...Addgene is poised to reach in the near future! We’re almost at: 100,000 different plasmids available from... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog Post... will expand the repository in ways that will be most useful to you, the members of our community. ... -
The 12 Days of CRISPR: 2021
TypeBlog Post...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration. On the tenth day of CRISPR, Addgene... -
It’s CRISPR Week at Addgene!
TypeBlog Post...CRISPR guide is a comprehensive resource that covers most things CRISPR - from nickases to base editing to... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post...April Fool’s Day on the Addgene blog. We’ve been mostly working remotely for the last year to give our ... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog Post...by Maki Naro 3) “Bird and Moon” by Rosemary Mosco 4) “Unearthed Comics” by Sara Zimmerman 5)... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post... suddenly. Please email [email protected] for the most-up-to-date information on shipping plasmids to your... -
Addgene at SfN23!
TypeBlog Post...conference blog posts before you go! Tips to Make the Most of a Scientific Conference Poster Session TipsHow... -
The Codon Guide to Synthetic Biology
TypeBlog Post... it regularly as people send me suggestions. The most useful parts of the Guide are probably the papers... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog Post...GIPHY We’ve been working hard to create the most informative and entertaining protocol and explainer... -
SciComm with the Experts at Science in the News Part 1
TypeBlog Post...breakdown Introduction to Vini, Amy, and SITN The Most Difficult thing Vini, Amy, and Outreach Scientist...