We narrowed to 567 results for: multi

1. Plasmids 101: Shuttle Vectors

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   Blog Post

   ...range plasmids are those that can replicate in multiple kinds of bacteria, including many natural plasmids...

2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...Resources Watch Dr. Michael Bassik’s lecture on “Multiplexing with CRISPR Screens”    ...

3. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   ...study profiled 38,731 cells from 694 embryos over multiple stages and reconstructed the patterns of gene ...

4. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ... the majority of astrocytes and neurons across multiple CNS regions. PHP.eB, an enhanced version of PHP.B...

5. Viral Production at Addgene

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   ...lentiviral vectors. This article was written by multiple Addgenies and most recently updated by Ina Ersing...

6. Viral Vectors 101: Preparing Pooled Libraries

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   ...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...

7. Fluorescent Proteins 101: Aptamer Fluorophores

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   ... ligands. References 1. Ma, Hanhui, et al. “Multicolor CRISPR labeling of chromosomal loci in human ...

8. Changing Labor Laws Bring Increased Postdoc Wages

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   ...be on higher salaries (which have been made by multiple organizations across academia). Increased postdoc...

9. The Challenges of Cell Culture

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   ...stocks of cells. Working stocks should be frozen in multiple vials and thawed to produce a new batch once you...

10. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...fluorescent microscope filter sets, users can label multiple proteins of interest with a combination of green...

11. An Integrin Antibody Toolkit from IPI

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    ...proved challenging to study. Cells often express multiple integrins that bind the same ligand, making it...

12. Adeno-associated Viruses (AAVs) for Genome Editing

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    ... alleles of a gene, or which require modifying multiple genes simultaneously in the same cell, are more...

13. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...GCN4 binding scFv that’s fused to GFP. When the multiple copies of the scFv-GFP fusion bind the SunTag ...

14. The Fluorescent Vegetables in Aptamer Soup

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    ... the only fruit-named aptamer, Mango, binds to multiple derivatives of thiazole orange (TO) and fluoresces...

15. Five Popular Model Organisms, Part 2

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    ...Hamdoun Lab to create fluorescent fusions in a multitude of organisms including Xenopus. Overall, the ability...

16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    ...influence whether cells will be co-transduced with multiple copies of both vectors. Examples of use: Halbert...

17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...vectors, the lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...

18. Fluorescent Proteins 101: When GFP lets you down

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    ...Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging." Proceedings of the National...

19. Harnessing Bacterial Toxins for Allelic Exchange

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    ...powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid curing steps, and can...

20. Tips for Getting a Faculty Position

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    Blog Post

    ... need a Nature/Cell/Science paper. You do need multiple first/corresponding author publications in quality...