We narrowed to 899 results for: plasmid

... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... 1μg of recipient plasmid. It is also critical that as much of the recipient plasmid as possible be cut...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

...copy regardless of plasmid size. See the plasmid's info page to determine if your plasmid is high or low ...resistance gene on a plasmid allows researchers to easily isolate bacteria containing that plasmid from bacteria...isolate your plasmid DNA from the bacterial culture by following the Isolating Your Plasmid DNA protocol... low copy plasmids? The copy number refers to the number of copies of an individual plasmid within a single...number plasmids should only need to be grown for 12–16 h on average. Certain features of a plasmid may render...Glycerol Stocks for Long-term Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture All Addgene...Culture Inoculating a Liquid Bacterial Culture Plasmids can carry one or more antibiotic resistance genes...

...Recovering Plasmid DNA from Bacterial Culture Recovering Plasmid DNA from Bacterial Culture You may also...concentrated plasmid DNA. This page will discuss the general procedure for purifying plasmid DNA from bacterial...perform plasmid purification without using a kit, you can find a protocol for kit-free plasmid mini-prep... the plasmid-containing supernatant. Add either ethanol or isopropanol to precipitate the plasmid DNA....high concentration of clean plasmid DNA. To improve the purity of plasmid DNA purified without a kit it...How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ Protocols... a new window) Promega sell kits for isolating plasmid DNA in quantities as low as a few micrograms to...

... also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation...both storing and replicating plasmids. Because of this, nearly all plasmids (even those designed for mammalian...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific ...tube of plasmid DNA and just need to transform bacteria so that you can grow up more of the plasmid) you ...efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea to use ...Learn how to transform E. coli with your plasmid of interest. Protocols...introduced into a cell. Transformation of bacteria with plasmids is important not only for studies in bacteria ...

...Addgene.org Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about...

...skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages ...

...photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here. In addition, it could be...GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins in ...

...localized fusion proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

...Resources Additional resources on the Addgene blog Plasmids 101: Aptamer FluorophoresAntibodies 101: scFVs...available from AddgeneBrowse our recombinant antibody plasmid collection References Grumezescu, A. M. (2018)....

...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer...

...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...

...stocks of their plasmids. This way, when you want to make more plasmid DNA, the plasmid will already be... Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids You may also...important for long-term storage of plasmids. Although you can store your plasmid DNA at -20°C, many labs also...also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Background Information... and growth temperature, can be found on your plasmid's information page. The next day you will be able...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration... glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid...

...flank the regions of your plasmid that you wish to confirm. Addgene's plasmid information pages recommend...Sequences" link on the Plasmid Information Pages. Addgene sequences the plasmid to verify tags, mutations...the entire plasmid. Addgene strongly recommends that you sequence any portion of the plasmid that is important...sequence analysis of a plasmid Protocols... Protocols Sequence Analysis Sequence Analysis of a Plasmid You may also like...Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion proteins...common primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for ...

...Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction ... commercial kit Plasmid Cloning Protocols for constructing and analyzing your plasmids. Name Description... that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...Intro to the Lab Bench Basic Molecular Biology Plasmid Cloning Virus Antibodies Intro to the Lab Bench...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification...spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis... the Video! Sequence Analysis Verify important plasmid features using sequence analysis RNA Extraction...

... Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety (see slides...is in one of the top 10 countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic...

...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...

...ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving away fun Addgene swag for...

...   Addgene is sharing so much more than plasmids these days. via GIPHY   You can see for yourself...

...DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your insert of interest...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ... cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be..., the plasmids should also be propagated in an endonuclease negative E. coli strain such as NEB stable...

... Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...