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We narrowed to 899 results for: plasmid

Showing: 521 - 540 of 899 results

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus...recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient plasmid by viewing... 1μg of recipient plasmid. It is also critical that as much of the recipient plasmid as possible be cut...ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid to insert. It...recipient plasmid + insert plate should have significantly more colonies than the recipient plasmid alone ... on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...

  3. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...

  4. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Storage of Plasmids Recovering Plasmid DNA from Bacterial Culture Background Information Plasmids can carry...copy regardless of plasmid size. See the plasmid's info page to determine if your plasmid is high or low ...resistance gene on a plasmids allows researchers to easily isolate bacteria containing that plasmid from bacteria... isolate your plasmid DNA from the bacterial culture by following Isolating Your Plasmid DNA . Antibiotic... low copy plasmids? The copy number refers to the number of copies of an individual plasmid within a single...number plasmids should only need to be grown for 12-16 hr on average. Certain features of a plasmid may ...clonal) colonies of bacteria carrying a specific plasmid. However, a liquid culture is capable of supporting...

  5. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Recovering Plasmid DNA from Bacterial Culture Recovering Plasmid DNA from Bacterial Culture You may also...concentrated plasmid DNA. This page will discuss the general procedure for purifying plasmid DNA from bacterial...perform plasmid purification without using a kit, you can find a protocol for kit-free plasmid mini-prep... the plasmid-containing supernatant. Add either ethanol or isopropanol to precipitate the plasmid DNA....high concentration of clean plasmid DNA. To improve the purity of plasmid DNA purified without a kit it...How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ Protocols... a new window) Promega sell kits for isolating plasmid DNA in quantities as low as a few micrograms to...

  6. Protocol - Bacterial Transformation

    Type
    Protocol
    ... also like... Plasmid Cloning by Restriction Enzyme Digest Restriction Digest of Plasmid DNA DNA Ligation...both storing and replicating plasmids. Because of this, nearly all plasmids (even those designed for mammalian...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific ...tube of plasmid DNA and just need to transform bacteria so that you can grow up more of the plasmid) you ...efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea to use ...Learn how to transform E. coli with your plasmid of interest. Protocols...introduced into a cell. Transformation of bacteria with plasmids is important not only for studies in bacteria ...

  7. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...stocks of their plasmids. This way, when you want to make more plasmid DNA, the plasmid will already be... Storage of Plasmids Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids You may also...important for long-term storage of plasmids. Although you can store your plasmid DNA at -20°C, many labs also...also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Background Information... and growth temperature, can be found on your plasmid's information page. The next day you will be able...able to start an overnight culture for plasmid DNA prep the following day. Tips and FAQ The optimal concentration... glycerol stock the same time you prepare your plasmid DNA. In the morning, when you retrieve your liquid...

  9. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...

  11. Addgene at SfN23!

    Type
    Blog Post
    ...ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving away fun Addgene swag for...

  12. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...flank the regions of your plasmid that you wish to confirm. Addgene's plasmid information pages recommend...Sequences" link on the Plasmid Information Pages. Addgene sequences the plasmid to verify tags, mutations...the entire plasmid. Addgene strongly recommends that you sequence any portion of the plasmid that is important...sequence analysis of a plasmid Protocols... Protocols Sequence Analysis Sequence Analysis of a Plasmid You may also like...Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion proteins...common primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for ...

  13. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...Amplify CRISPR pooled-plasmid libraries Diagnostic Restriction Digest Verify a plasmid using restriction ... commercial kit Plasmid Cloning Protocols for constructing and analyzing your plasmids. Name Description... that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...Intro to the Lab Bench Basic Molecular Biology Plasmid Cloning Virus Antibodies Intro to the Lab Bench...Bacterial Glycerol Stocks Store bacterial strains or plasmids for long term use Watch the Video! DNA Purification...spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel Electrophoresis... the Video! Sequence Analysis Verify important plasmid features using sequence analysis RNA Extraction...

  17. Lentivirus Production

    Type
    Protocol
    ...DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your insert of interest...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ... cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be..., the plasmids should also be propagated in an endonuclease negative E. coli strain such as NEB stable...
Showing: 521 - 540 of 899 results