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...high-throughput next generation sequencing (NGS). Select a restriction enzyme for digest that will cut the shared plasmid...visualize that digestion on an agarose gel ( see restriction digest protocol here ). Lentiviral plasmids can...closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing at 30 ℃...

...get all your burning AAV questions answered.  Interested? You can learn more here - and remember, submissions...

...all activity on X, formerly Twitter. If you are interested in engaging with us on social media, you can ...

...attention to the details. Chapter 3 - Answering interesting biological questions using microscopy. Chpater...

...continuously updating this page with new and interesting content. Wish we had a video on something that...

...Contributors of 2023 8 Great Depositor Research Stories Interested in contributing to the Addgene blog? We'd love...

...researchers who use VSV-G can deliver their genes of interest to a variety of cells and study their effects....

... Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. ...

... so we can add it to the table! And if you're interested in joining discussions about improving antibody...

...Introduction 1:01-9:04 : What got Professor Cepko Interested in Working on the Eye 9:05-10:16 : Cell Targeting...

...resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR Precise edits are difficult...

...Addgene Scientific Advisory Board with a particular interest in generating Cas9 based tools to enable facile...

...some great costumes this Friday. So if you’re interested in finding out who wins this year’s Addgene costume...

...#ChristmasScene #womenwhoscience #scigirls #wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication...

... further drill down into your specific area of interest. We also cover topics outside of these five areas...

...have any questions or useful insights. If you're interested in blogging for us, fill out our Guest Blogger...

...s laboratory at Harvard Medical School and is interested in improving CD8+ T cell responses to cancer....

...JEDI-2P, deposited by the St-Pierre lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2 (...

... a rousing success! Many of the students were interested to see so many different career options in science...

...complementary regions Note: If you will be including a restriction site at the 5’ end of your primer, note that ...the enzyme to cleave efficiently (e.g. GCGGCG-restriction site-your sequence). Protocol Video Watch the...