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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 759 results for: rest

Showing: 521 - 540 of 759 results

  1. Immunocytochemistry

    Type
    Protocol
    ...depending on the sample type and the protein of interest. You may need to try a variety of fixation methods...transfected with a plasmid to express the protein of interest, and a negative control sample such as cells that... that do not express the protein of interest....

  2. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...dithiothreitol, DTT), Invitrogen NP0009 PageRuler Plus Prestained protein ladder, Invitrogen 26619 Pipette tips...the hot plate to 100 °C. Thaw IgG standard and prestained protein ladder on ice. Reagent Preparation Prepare... that the chamber is sealed. Load 5 µL of the prestained protein ladder to the appropriate well. Pro-Tip...

  3. CRISPR Library Amplification

    Type
    Protocol
    ...high-throughput next generation sequencing (NGS). Select a restriction enzyme for digest that will cut the shared plasmid...visualize that digestion on an agarose gel ( see restriction digest protocol here ). Lentiviral plasmids can...closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing at 30 ℃...

  11. Call for Guest Bloggers

    Type
    Blog Post
    ... Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. ...

  13. CRISPR Cheat Sheet

    Type
    Blog Post
    ...resulting mutant cells are screened for phenotypes of interest. Limitations of CRISPR Precise edits are difficult...

  15. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...some great costumes this Friday. So if you’re interested in finding out who wins this year’s Addgene costume...

  18. New Viral Vectors - March 2024

    Type
    Blog Post
    ...JEDI-2P, deposited by the St-Pierre lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2 (...

  19. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...#ChristmasScene #womenwhoscience #scigirls #wearestemsquad #festivefun #phdlife #phdtips #sciencecommunication...

  20. Editor's Choice, July 2016

    Type
    Blog Post
    ...have any questions or useful insights. If you're interested in blogging for us, fill out our Guest Blogger...
Showing: 521 - 540 of 759 results