We narrowed to 538 results for: sequence
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TypeGuide...encodes the gene of interest and contains the sequences that will be incorporated into the host cell genome...2021). HIV-based lentiviral vectors: Origin and sequence differences. Molecular Therapy — Methods & Clinical... of the provirus. The U3 (unique 3’) contains sequences necessary for activation of viral genomic RNA ... repeat region. U3 in cis Unique 3’; contains sequences necessary for activation of viral genomic RNA ...the addition of a polyA tract just after the R sequence. WPRE in cis Woodchuck hepatitis virus post‐transcriptional...nuclear export. RRE in cis Rev Response Element; he sequence to which the Rev protein binds. Psi (Ѱ) in cis...
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Modular Cloning Guide
TypeGuide...DNA module is flanked with a standard overhang sequence at the restriction cut site, multiple modules ...containing basic parts (promoters, UTRs, coding sequences, terminators, etc.) flanked by fusion sites into...plasmids including regulatory elements, coding sequences, CRISPR parts GreenGate Cloning System Plant Expression... -
Educational Resources
TypeGuide...Readiness Molecular Biology Learn about plasmids, sequencing, vectors, and restriction enzymes to assist with... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...brief window in time “The most obvious future consequence of the undermining of the scientific process ...still is and will continue to be a “critical consequence of ...the marginalization of science,” we know...know now in the summer of 2020 that the consequences of undermining science are even more immediate and... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...the quantity of DNA that is required and, as a consequence, also dictate the required sizes of the bacterial...micrograms of plasmid DNA for each reaction. As a consequence, the preparations for this technique must be ... -
March for Science
TypeBlog Post...chosen to leave the lab and march. Unintended consequences While frustration and anxiety have inspired ...others are concerned about possible unintended consequences of a march. Three of these risks include: 1)... -
Antibody Guide
TypeGuide...antibody’s identity to be easily confirmed via sequencing, and enables the variable region and constant...the DNA is released from the complex and can be sequenced and amplified for analysis. Overview of application... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...dissociate due to conformational changes, and as a consequence, EB1 protein function is inactivated by the separation... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...current job as a Marie Curie Fellow is a direct consequence of a blog that I started writing three years ... -
Degrading DNA with Cascade-Cas3
TypeBlog Post...underutilized in research settings — mostly a consequence of Cascade's complexity. Figure 1: Schematic... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...and decreased functionality are all possible consequences of FP aggregation, as Erik Snapp’s blog post... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...that two FPs will collide with each other. The consequences can be dramatic. For a membrane POI localized... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...incorrect assumptions can be a direct cause or consequence of lapsed communication. Making some assumptions... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...the details of how each tool performs and the consequences of off-target activity. Whether you are studying... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...only fluorescence coming from this volume. The consequence is that you can detect signal in thick specimens... -
Optogenetics Guide
TypeGuide...TULIPs), plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags... -
Adenovirus Guide
TypeGuide...capacity AdV (HCAdV) vectors. Here, all viral sequences are deleted, except for the ITRs and the packaging... -
Neurodegeneration Plasmid Collection
TypeCollection...Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag...