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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 846 results for: tat

Showing: 521 - 540 of 846 results

  1. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...Although it was a huge innovation, there was a limitation to this approach. CBEs and ABEs are base transition...repair (BER), the cellular process that catches mutated U’s in DNA and ideally converts them back to Cs... Cas9s that recognize different PAMs. Their computational tool for predicting CGBE editing outcomes and...

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... had the lowest background activity in the dark state and highest fold induction at 31X. By using a slit...by blue light (470nm), paCas9-1 induced indel mutations via NHEJ (frequency of 20.5%) and induced modifications...significantly alter the system’s efficiency at generating mutations when activated with light and lowered background...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...sequence cells to verify excision didn’t introduce mutations Adenovirus Non-integrating Low Medium Generates...and/or oncogenes, potentially leading to the presentation of a cancer-like phenotype in infected cells...stringency test for mouse iPSCs, tetraploid complementation, involves injecting tetraploid blastocysts ...

  5. We’re Going Viral This Week!

    Type
    Blog Post
    ...Hub in this blog post. Alongside our efforts to facilitate the sharing of reagents and data, we’re also ... first paper came out last year on sucrose supplementation to increase AAV2 yields and we’re eager to ...

  6. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious Fulbright... : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition back...

  7. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ... community (check out the infographic below for stats on our plasmid deposits and requests). Over the ... the lookout for new ways to help researchers facilitate and accelerate scientific discovery. How can ...

  8. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...student? 4:50 - 8:00 : How did you approach doing rotations? 8:01 - 11:25 : How did you decide what lab to...50 : What is the number one question you think rotation students should ask senior graduate students? ...

  9. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...The recent sweeping cuts to science funding, limitations to facilities and indirect costs, and increased...scientific research ecosystem, not just in the United States but around the world. As an organization that directly...

  10. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...Addgenies! Our team recently grew big enough to necessitate an office move. We've got ~50 Addgenies working...with institutions and tech transfer offices that facilitate MTAs. The great feedback from scientists who ...

  13. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...then you'll find the plasmids listed below the citation. An example of these "Associated Plasmids" is ...increasing your work's visibility Help us improve our citations lists We are still growing our "Articles Citing...

  14. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ... in some form? There is a lot of talk about the state of scientist training in the US and around the world...postdoc-driven biotechnology clubs such as Penn State, Berkeley and Harvard. Students also partner with...

  15. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch to...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR...

  16. Science Communication for Everyone

    Type
    Blog Post
    ...sci-art that would ultimately give a visual representation of science to our audience. How can researchers...extensively use Google search. Be careful about hidden connotations behind a word. Non-scientists interpret terms...

  18. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...the Pacific Ocean, off of the coast of Washington state. Using these samples, he was able to isolate two... protein’s structure and also discovered many mutations that alter and improve its function and shift ...

  20. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...you need to, go to ESL classes Practice your presentation skills (as much as you can), form a peer mentoring...content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker committee...
Showing: 521 - 540 of 846 results