We narrowed to 794 results for: tec

...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...

...measurements such as patch clamp recordings. However, technical difficulties such as small fluorescence changes...QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials in worms with fluorescence changes...

...limitations. First, FRET signals are often difficult to detect, and thus quantify, due to small fluorescence changes...induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours, a time consistent...

...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...you stay connected. If possible, test out the technology before the conference begins.“There were some...

...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...

...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...

... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working...

...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated...

...contrast to recent genome engineering technologies, stem cell techniques are neither fast nor inexpensive....

...Culture by Gibco Whenever I start to learn a new technique, I try to look for a resource to help me. I’m .... When I started learning proper cell culture technique, I tried to find an app that could help me with...

...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest ...

...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant...

...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

...With increasingly sophisticated visualization techniques it is possible to follow the interactions between...microvillar search and stabilization during ligand detection by T cells." Science 356.6338 (2017): eaal3118...

...patient’s tumor sample, use cutting edge genomic technologies to map the mutations that are present, and use..., these early pilot studies have demonstrated technical feasibility and proof-of-concept. If such approaches...

...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...

...infrared fluorescent protein tag which let the team detect its co-localization with a membrane-localized mCherry...about using the secondary nanobody toolbox for immunodetection Resources on Addgene.org Find nanobody expression...

...into bacteria or eukaryotic cells is a common technique in molecular biology and scientific research. ...page, which has both protocols and videos for techniques in basic molecular biology, plasmid cloning, ...

...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...