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...to be sold by Clontech and is in a freezer box in just about every lab in the world, is not inert. In all...problem with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins fused to FPs that dimerize...

...which we will discuss later.       Standard curves must be included every time the assay is run, ideally...protocol is being followed.  Finally, standard curves must be validated for each run. To validate, include ...

... things contributed to the steadily increasing distrust the general public has in science and scientists...chapters in this section describe the challenges of trusting science in a “fake news and post-truth era,” the...

...tools designed to help reduce or eliminate bias, just like the ones made available through the work done...Paul S. Appelbaum, Bege Dauda, Anna Di Rienzo, Agustin Fuentes, Stephanie M. Fullerton et al. Getting ...

...to share it with others! After all, won't it work just as well for everyone else?  In truth, people are...Visually compelling and flexible. Can be used to illustrate things that cannot be seen with the naked eye...

...you would like to introduce the edit. Finally, you must stipulate what sequence to introduce, which is done...prior to and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within a day ...

...tend to be in the range of 1012 to 1013 GC/mL, you must serially dilute your virus before adding your sample...tricks for performing ddPCR Clean everything You must have a clean working area to titer your AAV by ddPCR...

...cells (Figure 1). The resulting HeLa cells show robust labeling, with strong expression and detection ...can be used to build these types of constructs robustly, quickly, and simply. We hope that you can similarly...

...interference), and CRISRPa (activation) libraries that do just that (Doench et al., 2016, Sanson et al., 2018).... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

...Ladder Ball... there might be a possibility it was just beginner’s luck, but I’m feeling optimistic for ...activity into the mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut...

...past 5-10 years, the Grants Profession has become just that, a profession. A grant professional works for... with research grants? Are you interested in sustainable community development? Volunteer as a grant writer...

...sometimes used as tags for detection; however, those are just one (admittedly large) class of common fusion protein...which a good antibody has not been developed (and just because your protein has a commercially available...

... with adeno-associated viruses (AAVs), will know just how tricky these tools can be to use. AAVs, which... from our plasmid lab to develop a reliable and robust high quality viral vector service, making an array...

... the detection antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that...development, however, can be challenging since scientists must first identify two independent antibodies that work...

...stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future... target cells (Figure 6).   Figure 6: Illustration of split fluorophore assay to test reconstitution...

...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

...rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence... SpCas9, indicating that the variants are likely just as selective as wild-type SpCas9. These VQR, EQR...

...editing treatment would have to be individually customized to the patient's specific mutations. With a drag... Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang,...

...place people share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide... idea into a viable solution.  Anticipate Change Just because a process is working, doesn’t mean it will...

...are 500–800 bp smaller than previous editors, but just as effective and with no additional off-target edits...on-target delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects...