We narrowed to 1,073 results for: TOR;

...are similar except that the vectors carrying the transdifferentiation factors are directly injected into... Kotton Four Factors that Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for... involves overexpression of four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated...another cell type of choice. The four reprogramming factors can be delivered and expressed in multiple somatic...supplements, bioactive small molecules, and growth factors are used to control the cell fate of iPSCs and ...         Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33... intermediate pluripotent state is not always obligatory in cell conversion processes [35]. Rather than...

...In-Fusion cloning molar ratio calculators Lab math calculators These calculators can help you with common...lab. Molarity calculator Promega's Biomath Calculators Primer resuspension calculator (sign-in required...data repository for validation data on Addgene/CLOVER viral vectors.  Caspedia: A data repository for ....)  DailyCalcs Science Calculator app for iPhone Reagent and Data Repositories These links can help you... for plasmid design and mapping. ApE (A plasmid Editor): A free, donation-based plasmid analysis tool ...enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...Golden Gate Reactions Cloning ligation reaction calculators These links can help you calculate how much insert...

...any viral vector. Addgene performs QC measures which verify the identity of the viral vector, but not ... Viral Vector Packaging Service Packaged on Request Scope of Service AAV Packaged on Request: Scope of...guarantees above. We will have our expert viral vector production team carefully review your cargo/serotype...

...Stable   For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences...derived. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences... HB101. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences...cells for cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled... your plasmid or carrying out your experiment. History of E. coli strains E. coli are gram-negative, rod... DH5alpha and DH10b (also known as TOP10). The history of B strain is a bit more convoluted due to researchers...researchers sharing and renaming samples throughout history. It was likely isolated in 1918 but was first referred...

...reaction to generate high-throughput targeting vectors. These vectors can be used to tag endogenous genes and...the final targeting vector (Fig. 1, Step 4), and co-injection of the targeting vector and a Cas9 expression...site destination vectors for inserting a tag at multiple target sites. SapTrap vectors can be easily modified...cells. To use CRISPaint, users need 3 vectors:  1) a gRNA vector that targets the gene of interest, 2)...PCR or cloning, as digestion of the destination vector with SapI yields 2 sites- the first site accepts...screening of the insertion event, and a variety of connector modules (linker sequences between the tag and ...with SapI allows the tag, selectable marker, and connector to be released (Fig. 1, Step 2-3). Since donor...

...are transfected with both vectors. Flex vectors are the solution. Flex vectors expressing fluorescent cytoplasmic.... It became possible to design FLEx vectors (Flip-excision vectors) that only allow one inversion event...concentrations of vectors expressing the required recombinase. The high concentration of FLEx vectors guarantees...experiments. Mutated FRT and loxP sites in Flex Vectors – inversion only once All recombination events ...between target sites of the same sequence in FLEx vectors, the DNA sequence that should be inverted is flanked... target sites are cleaved out, leaving behind a vector with the inverted DNA flanked by non-compatible...non-compatible target sites (Schnütgen et al. 2003). Flex vectors revolutionize neuroscience Manipulation of specific...

...has been exploited to localize factors such as transcriptional activators and repressors to target genes...target genes using dCas9-based activators The simplest of the dCas9 activators consists of dCas9 fused to ... dCas9 and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, generally ...promoter region. Cas9 activators include Cas9 fused to VP64, VP64 along with other SAM factors, and the Suntag...with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat. Biotechnol. 2020 387 38, ...array of Cas9s (many available through Addgene’s repository) and make it easy to find your perfect match....precise edit without even making a break.  Base editors are dead Cas9 (dCas9) or nCas9 enzymes fused to...

...imaging microscopy (FLIM) method. Acceptor photobleaching method The acceptor photobleaching method is simple...don’t measure acceptor fluorescence, this method is also less sensitive to direct acceptor excitation artifacts...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...fluorophore (the donor) to another fluorophore (the acceptor) by means of intermolecular long-range dipole-...dipole-dipole coupling." The emission of the acceptor fluorophore can be measured using microscopy techniques...photons) and the extinction coefficient of the acceptor (linking the quantity of absorbed light, at a ...highest quantum yield donor, the highest absorbing acceptor and fluorophores with significant overlap in their...

...responsible for storage.  If datasets are too large to transfer to a journal repository, they are likely...publishers. Nonetheless, the initial release caused a storm as the organization left open the question of how...for advice about the scope of the policy. End of story? Not quite. The PLOS policy on data access is a...statistical methods, omission of some data, etc.  The firestorm that followed implementation of the policy comprised... with limited acknowledgement of the initial contributors. This is probable to some extent - but that ...tools and assumptions to reveal findings that the originator was oblivious to, no matter how much time they...they had. It could be argued that data generators may, in light of the policy, hold onto data longer before...

... years ago and developed cloning vectors to harness it. These vectors, called pKIL18 and pKIL19, contained.... If, however, an investigator were to successfully clone an insert into the vector, the ccdB reading ...cells that contained non-recombinant vectors (re-ligated empty vectors, for instance) would still express...ccdB makes cloning easier by selecting against vectors that did not take up your insert. But exactly how...ccdB accomplish this? Let’s start with a brief history of the gene and how molecular biologists have harnessed...potent toxin... The ccdB gene, located on the F sex factor plasmid of E. coli, is part of a toxin-antitoxin...from Bernard, P., et al. showing the pKIL 18/19 vectors and demonstrating the concept of restriction enzyme-mediated...