...limitations. 3. Always provide value to others This is a non-profit organization. Therefore, collaboration and ... of how much you can expect to use in the future. 3. Identify the skills needed to use these tools Outline...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...

...back to the drawing board.  Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing social... Production Specialist, I’m constantly thinking about ways to improve our productions to deliver the best...four months after I started with Addgene, I was about to have a check-in with my boss Jennifer. I had ... would be a couple months. Jennifer and I spent about an hour that morning scrambling. Everything we had...someone to be comfortable on camera while thinking about things like lighting, camera angle, and shot composition... allowing people to sit without having to worry about getting in close to mic them up. Monitor: I then...first video in our Intro to the Lab Bench series about PPE! We made this with a combination of in-lab filming...

...the world — and we really do mean all over (Figure 3). We continue to add new posts every week, and we’...beautifully designed and easy-to-reference eBooks. Figure 3: Global readership of the Addgene blog in 2023. Views...a less tangible form: knowledge and information about the materials in our repository. We started to increase...by making it easier to find materials and learn about them. As early as 2007, we began grouping similar...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit...updating and expanding our website, we’ve learned about the importance of smaller, systemic changes, like...

...experimental design and gel quantification. Figure 3: In a perfect world, protein concentration in your...versus the amount of protein loaded on the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical... this chain (blue) to infer or calculate things about earlier ones. In practice, multiple sources of error...condition 2.” By eye, the loading control appears about the same between the two conditions. But it isn’.... However, it throws both assumptions discussed above into question. Assumption #1: The loading control...searching the literature to see what is already known about your housekeeping protein in your context. If you...step from the POI blot, you don’t need to worry about the linear detection ranges of multiple targets....

... originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA...PCR and a restriction enzyme digest.   Fig. 3: Restriction enzyme screening for resistivity to digestion... your edit, it’s important to know a few things about your target cells and the desired edits. First, ...et al., NAR). It works just like TIDE (outlined above), except that it uses a 3rd sequencing trace file...great option, especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing...higher probability locations. If you’re worried about off-target effects, you can consider using a version...SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with...

...conservative estimate. As a graduate student, I drank 3-4 cups of coffee a day, and I had colleagues who were...learn about all the amazing research being done with plasmids. I heard an impressive talk about the use...pulls off such an inspiring, thought-provoking, collaborative event – all while keeping the party going! At...scientists we've spoken with are excited and passionate about making such tools open-access. Though it is time...

... why they are the most commonly requested (Figure 3). Over 400 biosensors were distributed in 2023 alone... viral vectors that deliver GFP.   Figure 3: A) Cumulative AAV preps available at Addgene from...all, a delivery method. I would be remiss to talk about them without looking at what they’re delivering .... But in the years since, we’ve learned so much about what it takes to make these viral vectors not only...., 2016), video, educational resources, and collaborations, and will continue to do so, in the hopes that...leave a comment; or take our AAV survey. Learn more about GCaMPs and other tools developed through the GENIE...

... them. For intracellular targets, we usually test 3 different fix/perms (methanol, PFA-saponin, PFA-triton.... The approach we suggest is summarized in Figure 3. The principle is to confirm the antibody can detect...-use of an individual experiment.   Figure 3: Our recommendations for an approach that may be used...leukocytes. The file shared also contains information about the labeling pattern of several cell lines using...Laflamme, C., Virk, H., NeuroSGC/YCharOS/EDDU Collaborative Group, & ABIF Consortium. (2024). A guide to...

...startups, and real applications of SynBio. Chapter 3: Synthetic biology as the engineering of biology at...synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder...BioBuilder Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with...

...negative controls for genome-wide CRISPR screens; and 3) safe-targeting guides, which target genomic sites...PMID: 25513782. Pubmed Central PMCID: PMC4320685. 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease...following unanswered questions about CRISPR screen design. Unanswered questions about genome-wide CRISPR/Cas9...Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting ...