We narrowed to 997 results for: lars;

...another challenge associated with molecular plant parts, particularly for applied projects intended for...challenging to engineer a specific function in these multicellular organisms. However, the rewards are great if...biotechnology and provide a much needed boost to basic molecular plant research, more initiatives are needed to...associated with plant synthetic biology. Plant molecular biology has traditionally relied on overexpression...processes. Often the tools developed for plant molecular biology are specific to a given project, and like...pieces developed over the years were generated by large agricultural companies and carry associated intellectual...and the ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois ...

... tumor’s backstage team. Microenvironments are cellular regions with specialized structures. Tumors are...neuroendocrine, adipose, immune, inflammatory, angiogenic vascular, endothelial, and stromal cells - a rich soup ...promoting proteins, such as human growth factor, vascular endothelial growth factor, and interleukin-6 (...2009). Then, scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism...Centro Ciência Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing...BrbaraPinho6.      References Allinen, Minna, et al. "Molecular characterization of the tumor microenvironment...PMCID: PMC4788391. Kogure, Takayuki, et al. "Intercellular nanovesicle‐mediated microRNA transfer: A mechanism...

...Research Symposium organizer, David T. Riglar PhD. Here, Dr. Riglar discusses one of the six panel discussions...Many thanks to our guest blogger David T. Riglar! David T Riglar is a postdoc at Harvard Medical School.... problems that were identified last year.  In particular, at the symposium we will discuss where better...Jonathan Dinman, Chair of the Cell Biology and Molecular Genetics Department at University of Maryland ...collection about the scientific workforce, in particular about postdocs. Among other involvements, he ...science and public policy. Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing...

...sign up for Google Scholar alerts. Based on keywords that you provide, Google Scholar will send you daily...Johnna's New Under The Sun Blog and is part of her larger tutorial series, A Beginner’s Guide to Writing ...useful to make literature searching and reading a regular part of your routine. Set a goal to read a certain...engines for finding scientific literature are Google Scholar and PubMed. You can search keywords, titles, authors...given research area. Unless you are looking for a particular protocol or are trying to replicate an experiment...possibilities until the results focus in on a particular conclusion. Many papers aren’t always written...of sufficient quality (error bars, statistics, clarity in images etc)? Did they perform all of the appropriate...

...) is conceptually similar to the microRNA precursor mentioned above and is a popular RNAi trigger in the...exquisitely and robustly self-organize at the cellular and multicellular scale.     References Bezanilla M, Perroud...is fair to say that CRISPR has revolutionized molecular biology and has enabled functional studies in ...micro RNAs (amiRNAs) are frequently used. Although similar, amiRNAs are engineered from endogenous precursors...silencing plants. Non-destructive methods, most popularly with a fluorescent reporter, directly couple silencing...Institute where he investigated how plants achieve polarized cell growth in the lab of Luis Vidali. For his...10.1242/dev.186452 Wilson RC, Doudna JA (2013) Molecular Mechanisms of RNA Interference. Annu Rev Biophys...

...Restriction enzyme cloning is the workhorse of molecular cloning; however, one of its biggest limitations...The best applications for dsDNA inserts include: large insertions or deletions, including selectable DNA... sure to read the associated papers for their particular uses - *note* not all linked strains contain ...is to use mini-λ, a defective non-replicating, circular piece of phage DNA, that when introduced into ...generation of bacteriophage, which is not a common molecular biology technique. However, an advantage of this...Medicine at the University of Washington. She is particularly interested in science communication and in vitro...PubMed Central PMCID: PMC307639. 2. Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal ...

...is often the case with large genes. This is due to inefficient packaging of large inserts; a study by Kumar... While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges... deciding what packaging system is best for a particular experiment. The newer generation lentiviral packaging... guides) while a single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example...dishes or increasing the vessel size. Opting for a larger vessel, such as a 3 or 5-layer flask will require...calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and adjusting the cell...start. If you don’t anticipate that you will need large volumes of lentivirus routinely, you may opt for...

...improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum et al., 2014, Konermann...Konermann et al., 2015, Chavez et al., 2015). Most popular methods for CRISPR activation CRISPR activation ...compared to other CRISPR activators, making it a popular method for gene activation experiments. In cases...exhibits activation levels comparable to other popular activation methods (VPR and SunTag) (Chavez et ...relies on antibody chains, which are relatively large and are not expressed consistently throughout cells...compared to the SAM system. Its activation levels are similar to that of SunTag. An advantage to this method ...While SAM, SunTag, and VPR have become the most popular methods for CRISPR activation, research in the ...

...vertical.  Large central box. Another commonly used and effective style is one employing a large central ... figures fit nicely into circular shapes rather than a square or rectangular boxes. Background and color...poster together, be aware that there are some particular styles recognized to work well. It is important...is written across the center of the poster in a large font. Hence, spectators can read the text from many...written and graphical information are inserted into larger circles or ovals, that are arranged in some logical...these colors so that your text is easily read. Similarly, be cognizant of audience members that are color-blind...

... and circular assemblies. While Golden Gate Assembly worked for all the assembly types, circular assemblies... 52-piece circular assembly of the T7 genome had lower efficiency than the 10-piece circular assembly,...rules the best way to design GGA overhang sets, particularly for high-complexity reactions? The researchers...outlined below, allow for researchers to select large sets (with overhangs that violate some of the traditional... use to identify fusion sequences for either a circular or linear assembly. If you aren’t constricted ...fairly easy cloning technique (which may be of particular interest to MoClo users!). If you’re interested...