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We narrowed to 1,041 results for: ins

Showing: 541 - 560 of 1041 results

  1. Editor's Choice, September 2016

    Type
    Blog Post
    ...Technique: Probe Phage Genomes for Host Binding Proteins & Running for Rare Disease, Running for FOP, Running...Technique: Probe Phage Genomes for Host Binding Proteins," was contributed by guest blogger Jessica Sacher...describes a technique you can use to find phage proteins that bind to your bacterium of interest. For those...an ingenious but simple technique to screen for proteins that could be useful for a wide variety of reasons...

  2. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to...Education and Development workshop aimed at brainstorming strategies for using combinations of AAV-based... to the target cell’s surface proteins and this interaction determines the specificity and efficiency ...

  3. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ... suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from rare diseases...Through their BeHEARD Award, the Rare Genomics Institute partners with companies to provide technological...Elia Di Schiavi is a Research Scientist at the Institute of Biosciences and Bioresources in Naples, Italy...interactions with other genes. He will use fluorescent proteins to monitor the localization of these genes, CRISPR...

  4. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    ...evaluate the impact of their work (H-index for instance), but these rely upon other researchers being ...plasmids that can be used for specific purposes. For instance, on our Optogenetics Collection page, you can ...containing plasmids for expressing different types of rhodopsins and sensors as well as references and links to... Blog Post on Our Citations Feature Browse More Inside Addgene Posts Additional Resources on Addgene....

  5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...the presence of scientific heroines is always inspiring. I was especially excited to attend this event...genome. Their award acceptance addresses were inspiring! We also got to hear more from them during a panel...especially thanked Martin Jinek and Krzysztof Chylinski (who coincidentally speak the same Polish sub-...to generate mice with genes knocked out in the insulin signaling cascades in only 4 months compared to...

  6. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...recycled. This service does cost money, but your institute may still be interested as it offsets the cost...how to start a glove recycling program at your institute! What should I do in the lab to help the environment...back. Start a glove recycling program at your institute! Universities and companies have the potential...posts and updates from Labconscious. If you’re inspired by this post then I challenge you to take to action...

  7. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...does increase the chance of false-positives, as proteins may appear to be binding at particular genomic...Nuclei are then incubated with a primary antibody against the protein of interest. The Protein A fused MNase...G (IgG) thus targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker...interactions Learn about other types of fusion proteins Study chromatin interactions with CAPTURE Additional...

  8. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any scientist’s...and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide to becoming...Di Rita, and, last, homework assignments. The instructors are leaders in the field of microbiology with...writing everyday Overall, the most important instruction the classes taught me was to write every day....

  9. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...and Leaders. I like her use of the term “cycles” instead of levels because it emphasizes the importance ...started and then step back to let others take the reins. They work alone much of the time, depending on ...it is the leader’s job to provide the driving inspiration, to create a productive culture and to correct... by Peter Fuda and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals...

  10. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow...purify it – and you do not have a specific antibody against the protein available – you can always add an epitope...mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection cassette for growth... collection of vectors carrying several fusion proteins. Pick your promoter, selection cassette and visualization...

  11. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...have recently received a grant from the National Institutes of Health that will address this problem.The ...produce a subset of this collection as ready-to-use-proteins to facilitate use. Dr. Trimmer’s lab will ensure... represent the official views of the National Institutes of Health.     References and Resources References...10.1038/520295b Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...

  12. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...of the Ocular Genomics Institute, and an Associate Member at the Broad Institute of Harvard and MIT. ...In 11th grade she heard a talk about Z DNA that inspired her to major in molecular biology in college. ... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...

  13. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...communication graduates are often well-equipped. Many institutions are sincerely interested in connecting with ...the experimental aspect of my thesis: a survey instrument designed to measure how individuals respond to...science. Understanding just how the public utilizes insights gleaned from scientific research is, after all... this power and the tools to deploy its wisdom remains a challenge. Departments like ours reflect a growing...

  14. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...locus or more commonly via cloning of recombinant proteins. Why use a tag? If you are fortunate enough to...may not need to read this - but for many other proteins, quality antibodies do not exist, or they don’...benefits – as discussed above – but there are a few instances when they should be used with caution or not at...protein may disrupt the biological function of some proteins. Protein folding issues, steric hindrances, and...

  15. Plasmid Cloning by PCR

    Type
    Blog Post
    ... use similar processes to add overhangs to your insert of interest for Gibson assembly. The steps following...to choose enzymes that: Do not cut within your insert Are in the desired location in your recipient...our cDNA into the recipient plasmid. Remember to insert your DNA in the correct orientation in the recipient...cloning Run the PCR reaction Run PCR to amplify your insert DNA. It is important to use a high fidelity polymerase...

  16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...secondary structure that can bind specific proteins via aptamer domains. Combined with dCas9, this new scaffold... sites. With hundreds of characterized aptamer domains available, an unprecedented level of flexible tunability... Designing and using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797-1807 (2012...

  17. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...As MIT's Ed Boyden explains it, his goal is "to understand the brain at a level of abstraction that enables...opsin from algae that is sensitive to red light instead of blue, which will allow researchers to independently...cells within them work together or perhaps compete against each other. Getting two-color stimulation has been...maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other conditions....

  18. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... hands-on, it will be “brains-on”! I know that even lectures should be “brains-on”, but as much as I try...received a whole set of cards (Promoters, Inserts, Tags, Origins of Replication, Resistance gene and Selection...

  19. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...organisms. However, lengthy genetic payloads and insufficient transcription on the array have limited the ...editing. How do you then set up an MBE system that contains a double-win for both efficiency and multiplexity...lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases, such as muscular dystrophies...CRISPR-based gene editors, for correcting mutations or installing functional genes to study and potentially treat...

  20. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...and are not used for general plasmid selection. Instead, reporter genes are often employed to easily monitor...of picking up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a...picking up of a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a... should not be used for mammalian expression – instead use G418. This can be confusing since the neo/kan...
Showing: 541 - 560 of 1041 results