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We narrowed to 143 results for: ins

Showing: 1 - 20 of 143 results

  1. Institute for Protein Innovation

    Type
    Collection
    ...ligands (glypicans and integrins), components in the synapse (neurexins and neuroligins), and common epitope...synaptic cleft proteins are currently under development. Figure 1: Key adhesion proteins and receptor–ligand...Reactivity Recommended Applications Return to top Integrins Integrins are a family of mechanotransduction receptors...and secreted proteins from a collaboration between Addgene and IPI. Antibodies... Antibodies Institute for Protein Innovation... Innovation Antibody Collection Institute for Protein Innovation Antibody Collection Neurexin/Neuroligin...Plasmids (Link opens in a new window) Welcome to the Institute for Protein Innovation (IPI) Collection! Here,...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...conformational changes. Organic dyes, fluorescent proteins, and other molecules have been demonstrated for...Pairs This table lists some popular fluorescent proteins used as FRET pairs along with their properties...calibration standards consisting of donor and acceptor proteins fused with linkers of varying lengths. Description...Description Article ECFP-EYFP FRET standards, fusion proteins with linkers of varying length Evers et al. (2006...understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers mCerulean...mTurquoise2-mVenus FRET calibration standards and fusion proteins Feldmann et al. (2023) Protocol for deriving proximity...

  3. Synthetic Biology - Strains

    Type
    Collection
    ...Signaling Strains Browse Addgene's collection of synthetic biology related bacterial strains. SynBio Bacterial...Strains for use in Synthetic Biology. Plasmid... Plasmid Collections Synthetic Biology Bacterial Strains Synthetic...Synthetic Biology: Bacterial Strains SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio... Bacterial Strains Search the table by keyword or sort by the table headings. Click on the publication... this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication Back to Top...

  4. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion of ...utilizing one of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This...Cloning Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid...R., Abraham, J., Hensley, A., Jones, K. L., & Brzezinski, J. A. (2021). Initiation of Otx2 expression ...Goodson, N. B., Kaufman, M. A., Park, K. U., & Brzezinski, J. A. (2020). Simultaneous deletion of Prdm1...

  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Depositor Collections Allen Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid...Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science has produced the first publicly... will be able to generate fluorescently tagged proteins of key cellular structures in human cell lines...additional cell line information from the Allen Institute for Cell Science data portal (Link opens in a ...cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP AICSDP...114406 SMC1A-mEGFP AICSDP-57 mEGFP SMC protein 1A Cohesins 114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin...

  6. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ... Allen Institute for Brain Science AAV Enhancer Collection You May Also Like... Allen Institute for Brain... from Allen Institute's Genetic Tools Atlas (Link opens in a new window) . Allen Institute for Brain Science...Enhancer Plasmids The Allen Institute for Brain Science Enhancer Collection contains best-in-class AAV plasmids...cell populations were tested in mouse brains. This collection contains plasmids for cell populations in the...created by the Allen Institute for Brain Science. Depositor... Depositor Collections Allen Institute for Brain Science...drive cell type-specific expression. The Allen Institute for Brain Sciences has screened thousands of enhancers...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's L. Miguel...Miguel Martins 14124 pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2...Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's L. Miguel Martins 15940 pcDNA3-HtrA2...Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's L. Miguel Martins 16155 pET20b-...His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-...

  8. CRISPR Guide

    Type
    Collection
    ...inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both... edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor DNA at “...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...

  9. Antibody Guide

    Type
    Collection
    ...four protein chains: two large heavy chains and two small light chains. These four domains come together...their monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin ...ankyrin repeat proteins. These are antibody mimics instead of modified antibodies and are not closely related...synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead of the antibody...Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE gel to separate proteins by size...your antibody’s specificity. A blank (contains water or buffer instead of a biological sample), which captures... lab to specifically and tightly bind to other proteins. Antibodies, produced as part of the immune system...

  10. Bacterial Expression Systems

    Type
    Collection
    ...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the culture...recombinant proteins to the periplasmic space between the inner and outer membranes. Targeting proteins to the...Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living or fixed...Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent ...In BiFC, proteins of interest are fused to each part of a split FP. When the candidate proteins interact...Luciferase Fluorescent Biosensors Chromoproteins: Colorful Proteins For Molecular Biology Experiments ...Consortium page contains a variety of reagents for engineering and expressing zinc finger proteins. Addgene ...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...Firefly Insertion of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5'...Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly Insertion of target... Renilla Insertion of target sequence in the 3' UTR David Bartel 12177 pIS2 Renilla Insertion of target...construct that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return ...Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion proteins for bioluminescent chemo-optogenetic...organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate...regions, or shRNA or CRISPR target sites can be inserted into a luciferase-containing plasmid and the effects...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ... E MGC4638 INS insulin ILPR, IRDN INS-IGF2 INS-IGF2 readthrough transcript - INSL3 insulin-like 3 (Leydig...MGC119819, RLF, RLNL INSL4 insulin-like 4 (placenta) EPIL, PLACENTIN INSL5 insulin-like 5 MGC126695, MGC126697...MGC126697, PRO182, UNQ156 INSL6 insulin-like 6 RIF1 INSR insulin receptor CD220, HHF5 JAG1 jagged 1 (Alagille...communication between cells of the immune system. Interleukins Interleukins are cytokines that are secreted by immune... omega 1 - IGF1 insulin-like growth factor 1 (somatomedin C) IGF1A, IGFI IGF1R insulin-like growth factor...MGC18216 IGF2 insulin-like growth factor 2 (somatomedin A) C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974 IGF2R...bA12D24.1.1, bA12D24.1.2 RLN3 relaxin 3 H3, RXN3, ZINS4, insl7 RNASE2 ribonuclease, RNase A family, 2 (liver...

  13. Optogenetics Plasmid Collection

    Type
    Collection
    ...microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins, are pumps...encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins, which...Find optogenetics plasmids such as microbial opsins and optical switch tools to activate, inhibit, and... opened and closed in response to light. These proteins can be expressed in specific neurons, often utilizing...in a precise manner. One of the first microbial opsins to be adopted as an optogenetic tool is the widely...anions into or out of the cell. When using these opsins as a tool to control neuronal activity, scientists...expression and localization. Many other types of proteins can also be controlled by light, generally by ...

  14. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mCherry tagged fusion proteins in worms ins:MCS; cryaa:RFP - A zebrafish insulin promoter vector with ...designed for a user to insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning...and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization...backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more. Plasmid...molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow...function section for if you want to stably express an insert using viral vectors, are studying a different genetic...mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...utilized CRISPR as a powerful defensive strategy against viral invaders, and this system is proving to be...genome in 1987, but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists...re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci to generate...system. Within the bacterial genome, a CRISPR array contains many unique protospacer sequences that have homology... potential target sequence is only valid if it contains a special Protospacer Adjacent Motif (PAM) directly... varying PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types...

  16. Zhang Lab CRISPR Page

    Type
    Collection
    ...loops at tetraloop and stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids...2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial...nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression cassettes. To target a given ...NGG PAM sequence. 3. SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized...2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized...SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Mullineaux Insulin Sensor for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...Additional Resources Fluorescent biosensors are proteins designed so that their fluorescent properties ...transfer (FRET) efficiency of a pair of fluorescent proteins. Genetically encoded biosensors can be introduced...Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul...nested reporters of green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested...cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer...

  18. Antibody Plasmid Collection

    Type
    Collection
    ... monoclonal antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount...of membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies against delicate proteins...Antibodies Antibody Plasmids Antibodies are large proteins made by the immune system that bind to an antigen...pull out a protein of choice from a mix of other proteins, visualize a protein under a microscope, or detect...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots... Antibodies: Selecting and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology...to rapidly engineer the constant immunoglobulin domains to obtain recombinant hybridomas, which secrete...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ...nerve cell function within the cell. Parkinson's Disease Parkinson’s disease (PD) is a chronic and progressive...to highly conserved proteins and other difficult-to-target antigens in the Institute for Protein Innovation...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including Markers...Differentiation Factors Antibodies Fluorescent Proteins Other Resources Neurodegenerative Disorders Neurodegenerative...neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease. These diseases...with the mutated HTT gene will have huntingtin proteins with abnormally long polyglutamine sequences. ...organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions with FUS and HNRNPA1...

  20. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...or MCP tagged plasmids for visualization of proteins inside cells....-, ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated... labeling cell-surface proteins, and are useful for labeling secreted proteins with disulfide bridges ...features: Allows for visualization of multiple proteins simultaneously with dual labeling when used with...live or fixed cells, and for internal or surface proteins Single constructs are compatible with multiple... and MCP-tags are used exclusively for surface proteins and use enzymatic binding to attach substrate ...suitable for both cell-permeant and non-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to...
Showing: 1 - 20 of 143 results