We narrowed to 1,015 results for: LEA

...to specific subcellular locations, such as the nuclear envelope, allowing scientists to probe interactions...expression levels but for many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized...

...loop-structure (Querido and Chartrand, 2008). Finally, a nuclease-deficient Cas9 can bind loci in the genome that...interfere with the function of the protein. This is clearly the case when proteins undergo post-translational...

... in the classroom on our blog. Now that you’ve learned more about popular model organisms in the lab, ... about plasmids in our Plasmids 101 blog series Learn more about CRISPR and genome editing in our CRISPR...

...tip: When designing your PCR, ensure there is at least ~200 base pairs of sequence flanking the editing...find a null clone, you should screen, at the very least, 4 clones. Did you use a dual guide system to generate...

...governments exist in reality and science is how we learn about reality. Formally, a government can be defined...public discourse, many scientists have chosen to leave the lab and march. Unintended consequences While...

...multiplexing gRNAs here). Cas9 is commonly tagged with a nuclear localization sequence to enhance targeting to the...plant species or cell type (Belhaj et al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9...

...(7). This modification allowed the deletion of release factor 1, the protein responsible for terminating...reference and protocols for use of this system, please reference ‘Rinehart Lab reagents for improved expression...

...fungus Neurospora crassa. The QUAS promoter is less leaky than UAS in the basal state, and co-expression of...expression. Co-expression of repressor QS along with QF leads to intermediate expression from QUAS, as seen with...

...Mentoring Program Director of Strategic Initiatives. Learn more at https://www.linkedin.com/in/lopezdiegothinkbrain...lopezdiegothinkbrain        Other Resources Visit the IMP website Learn about the Mentor Circle Program at Brigham and Women's...

...time investment. Once the target mRNA is cleaved, the cleaved mRNA can induce further rounds of RNAi. ...

...References 1. Hochedlinger, K. and R. Jaenisch, Nuclear reprogramming and pluripotency. Nature, 2006. 441... Factors that Differentiate the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery...

...used in this approach should also allow for the clear identification of the cell types of interest. By...Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A....

...Ready to learn about antibodies? Our latest animation is here to help! Join Abi as they explain what ...

... to take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia; they...

...fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during the school year...

...second edition includes new and updated content in a clean and easy-to-navigate design. You can find it, along...

... "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies animation has...

...burning AAV questions answered.  Interested? You can learn more here - and remember, submissions are due Nov...

...here Additional resources on the Addgene blog Learn more about Blugene's make-over Get a how-to guide...

...BioRender.com. Figure 1: PRIME editing Please check out the updated guide for a great introduction...

...episode of our Hot Plasmids podcast series, you'll learn about new libraries for studying B. subtilis biology...

...episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins, AAV tools for...

... nonprofit. Recent remarks and actions by X’s leadership conflict with our values. For this reason, our...

...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene gave to ...

... Denise Roche, to edit the translation. We are pleased to announce that CRISPR 101: Una herramienta de...

...photos of our pets with Addgene gear, and have unleashed them all over our recently redesigned blog. 🐕...

...Ahem, ahem, drum roll please! It's time to announce the winners of our 2021 Deck the Lab Contest! Though...

...to help you with your CRISPR experiments and to learn about new CRISPR tools. Since the CRISPR field keeps...

...difficulties of getting a lab up and running, and learn a little bit about what it was like for Wei Leong...

...create a new fold that is not present in nature (at least that we’ve seen so far!). They started by designing...