Addgene

We narrowed to 925 results for: TIM

Showing: 811 - 840 of 925 results

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Viral Vectors 101: Viral Vector Elements

Type

Blog Post

...used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and...
Viral Vectors 101: Types of viruses

Type

Blog Post

...lab? Well, they are essentially evolutionarily optimized gene delivery devices. Their survival depends ...
Viral Vectors 101: Viral Applications

Type

Blog Post

...simultaneously in your system instead of just one at a time. Many of these libraries are packaged and delivered...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

...their low immunogenicity, which means they do not stimulate a strong immune response when introduced into ...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

...post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes via increased nuclear...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

... may be undesirable in many applications. At the time of our first blog post on choosing a Cas9, the Feng...
How Addgene Creates Content

Type

Blog Post

...in the areas in which they have expertise. Other times, we may need to reach out to an external expert ...
Plasmids 101: Yeast Vectors

Type

Blog Post

...replication of these vectors. S. pombe plasmids oftentimes utilize an ARS to aid in high transformation ...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...inserting electrodes into the brain. But this process is time consuming and expensive. Thus scientists have turned...
Antibodies 101: Fab Fragments

Type

Blog Post

...affinity reagents, and for good reason. However, sometimes you want a tool that is a little more compact ...
Antibodies 101: Conjugation

Type

Blog Post

...antibodies are provided in a buffer with the antimicrobial agent sodium azide, which can interfere with...
Transferable Skills Guide: Cross-team Communication

Type

Blog Post

... protocols, SOPs, and methods sections. The next time you write or read one of these, keep an eye out ...
It’s Bioelectric! An Exciting Interdisciplinary Field of Research

Type

Blog Post

... light (590nm). BLINK2 potassium channel - An optimized blue-light induced synthetic potassium channel...
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Type

Blog Post

...cumbersome, can lower on-target efficiency, and sometimes even increase off-target effects, respectively...
The Future of Research Symposium Boston 2015

Type

Blog Post

... and students will again gather in Boston - this time with the aim of finding solutions to some of the...
Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

...genome of an hiPS cell. While the majority of the time the genome will attempt to repair itself without...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

...the MTA. Pro Tip! Download a new spreadsheet each time you want to submit new plasmids! We update the spreadsheet...
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Type

Blog Post

...treat these diseases (drug repositioning). The ultimate goal of this work is to lower the burden of immune...
Educational Planarian Tools for Teaching Developmental Biology Techniques

Type

Blog Post

... for viewing how gene expression changes through time or after genetic manipulation. The technique involves...
Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Type

Blog Post

... interact with the bait at a particular point in time, i.e. when the bait is interacting with a particular...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

...the transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...this TIR1 complex binds to the degron sequence ultimately leading to the degradation of the AID-tagged ...
Hot Plasmids and Viral Preps - January 2021

Type

Blog Post

...experiments that require high photostability, such as time-lapse imaging or with high light power detection...
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Type

Blog Post

...cells. While editing single genes is very useful, sometimes, you need to modify multiple genes to generate...
Synthetic promoter AAVs for cell-type specific expression in retinal cells

Type

Blog Post

...genes in particular tissues, but they are costly and time-consuming to generate, especially for large animals...
Viral Vectors 101: Calcium Sensors

Type

Blog Post

...in vivo studies. There are different versions optimized for neuron-specific, astrocyte-specific (GCaMP6...
Viral Vectors 101: Virus Safety

Type

Blog Post

...1. In BSL-2 labs, eye protection is required any time you have to work with BSL-2 materials, such as lentivirus...
Antibodies 101: Choosing the Right Isotype

Type

Blog Post

...greater amount of antigen in a shorter period of time and would likely perform better for probing poorly...
Progress Towards a PAM-Free CRISPR

Type

Blog Post

...desired edit to still occur if the cut site is sub-optimal? As with everything in biology, the answer is always...
Hot Plasmids February 2024

Type

Blog Post

... The Junwei Shi Lab addressed this problem by optimizing the Cas protein delivery with Peptide-Assisted...

Showing: 811 - 840 of 925 results

Results per page:

Viral Vectors 101: Viral Vector Elements

Viral Vectors 101: Types of viruses

Viral Vectors 101: Viral Applications

Viral Vectors 101: Viruses as Biological Tools

Viral Vectors 101: Gamma-Retroviral Packaging Systems

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

How Addgene Creates Content

Plasmids 101: Yeast Vectors

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Antibodies 101: Fab Fragments

Antibodies 101: Conjugation

Transferable Skills Guide: Cross-team Communication

It’s Bioelectric! An Exciting Interdisciplinary Field of Research

Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

The Future of Research Symposium Boston 2015

Plasmids for Endogenous Gene Tagging in Human Cells

Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Educational Planarian Tools for Teaching Developmental Biology Techniques

Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Mouse Modeling, Part 1: Genetically Engineered Mice

Hot Plasmids and Viral Preps - March 2021

Hot Plasmids and Viral Preps - January 2021

A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

Synthetic promoter AAVs for cell-type specific expression in retinal cells

Viral Vectors 101: Calcium Sensors

Viral Vectors 101: Virus Safety

Antibodies 101: Choosing the Right Isotype

Progress Towards a PAM-Free CRISPR

Hot Plasmids February 2024

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