Addgene

We narrowed to 836 results for: Tat

Showing: 811 - 836 of 836 results

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Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Type

Blog Post

...method for converting heterozygous to homozygous mutations. Science aaa5945 (2015). doi:10.1126/science.aaa5945...
Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

... on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies to Control Protein Activity...
Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

...scientists' transferrable skills. In describing the limitations of the study, the authors noted that “Many trainees...
Antibodies 101: Producing Recombinant Antibodies

Type

Blog Post

...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

Type

Blog Post

...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop...
The Importance of a Fun Workplace: Company Culture at Addgene

Type

Blog Post

...environment Team building events like Escape the Room or Habitat for Humanity take you and your co-workers out of...
Negotiating Work and Life: How to Find the Joy

Type

Blog Post

...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

... PHP.B and the C57BL/6J version of LY6A, but a mutation in PHP.B’s unique capsid loop disrupted binding...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

... they can understand and are engaged by your presentation, then you’re on the right track. Furthermore...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Type

Blog Post

... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers...
Healthcare Consulting: A Door to the Business of Life Sciences

Type

Blog Post

...pointed feedback or abruptly change needs and expectations. Such situations can get stressful and to help...
Hot Plasmids - August 2020

Type

Blog Post

...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...was certainly an issue worth addressing. Clear expectations have been established for future participants...
An Inside Look into Addgene's Dev Team

Type

Blog Post

...provides transparency to everyone involved about the status of each project. This creates accountability on...
Viral Vectors 101: Pseudotyping

Type

Blog Post

... Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for enveloped...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

...to the research community. It does, however, necessitate us first identifying broadly useful vectors, ...
How to Be an Excellent Trainee

Type

Blog Post

...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage...
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...
CRISPR 101: Any Base Transversion Editors

Type

Blog Post

... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

...darker blue indicates more views from that country. Stats and figures are helpful, but our favorite measure...
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Type

Blog Post

...one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination is necessary...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

...intracellular T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic ...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

...cross-linking, killing the cell but preserving the cellular state (think of mummification). Permeabilization, as the...
Special Delivery: Fluorophore Targeting for FRET Studies

Type

Blog Post

...PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved cameleons."...

Showing: 811 - 836 of 836 results

Results per page:

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Plasmids 101: Secondary Nanobody Toolbox

Transferable Skills Guide: Identifying Your Transferable Skills

Antibodies 101: Producing Recombinant Antibodies

CRISPR 101: Ribonucleoprotein (RNP) Delivery

Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

The Importance of a Fun Workplace: Company Culture at Addgene

Negotiating Work and Life: How to Find the Joy

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Designing Your Chalk Talk for the Academic Job Interview

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Healthcare Consulting: A Door to the Business of Life Sciences

Hot Plasmids - August 2020

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

An Inside Look into Addgene's Dev Team

Viral Vectors 101: Pseudotyping

Twenty Years of Sharing: Addgene's Viral Vector Service

How to Be an Excellent Trainee

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

CRISPR 101: Any Base Transversion Editors

Twenty Years of Sharing: Expanding Accessibility

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Antibodies 101: Single Chain Fragment Variables (scFvs)

Antibodies 101: Beyond Surface Labeling

Special Delivery: Fluorophore Targeting for FRET Studies

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