We narrowed to 844 results for: Tat

... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

...finally, to expert.   Figure 1. Visual representation of common injection routes in mice. Image created...

...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

...sequence of DNA. Second, NgAgo target specificity is dictated by a DNA guide sequence rather than an RNA guide...

...method for converting heterozygous to homozygous mutations. Science aaa5945 (2015). doi:10.1126/science.aaa5945...

...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....

..., routine machine maintenance, and critical interpretation of the results.  If you see evidence of contamination...

... on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies to Control Protein Activity...

...scientists' transferrable skills. In describing the limitations of the study, the authors noted that “Many trainees...

...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop...

...environment Team building events like Escape the Room or Habitat for Humanity take you and your co-workers out of...

...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

... PHP.B and the C57BL/6J version of LY6A, but a mutation in PHP.B’s unique capsid loop disrupted binding...

... they can understand and are engaged by your presentation, then you’re on the right track. Furthermore...

... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers...

...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

...one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion ...

...darker blue indicates more views from that country. Stats and figures are helpful, but our favorite measure...

...to the research community. It does, however, necessitate us first identifying broadly useful vectors, ...

...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage...

...time-consuming, depending on the library prep and instrumentation used.  Here at Addgene, we sequence every plasmid...

...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ...

... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product...

... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...

... Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for enveloped...

...was certainly an issue worth addressing. Clear expectations have been established for future participants...

...provides transparency to everyone involved about the status of each project. This creates accountability on...

...pointed feedback or abruptly change needs and expectations. Such situations can get stressful and to help...

...truncated, non-fluorescent form of YFP as the default state of the transgene such that recombination is necessary...