We narrowed to 1,025 results for: URE
-
TypeBlog Post..., you will be able to search for your favorite features, such as serotype, promoter, or tool type. Drilling...accepting data for materials distributed by Addgene. Future directions As the AAV Data Hub grows, we hope to...
-
Top Requested Plasmid of 2017 - pMD2.G
TypeBlog Post...guide page. For more protocols and information, be sure to check out the Trono Lab website. If you have ... plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours... -
Deck the Lab 2022!
TypeBlog Post...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...@seleena78) Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...Fun stats and trivia begins! Tuesday: Depositor features (plasmid deposits and blog posts showcasing the...year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach ~14,000 plasmids deposited... -
Our New Antibody Mascot is....
TypeBlog Post... Fig. 1: Meet...well, frankly, we're not sure yet! This as-yet–unnamed friend is a huge ...mascot, an antibody joining us on our scientific adventures. You can submit your name suggestions here or... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post... purity (260/280 nm and 260/230 nm ratios) and measure the concentration. Since this is a beta project...you’d like to see as part of the service in the future using the Cloning Grade DNA Suggestion Form. ... -
Congratulations, Deck The Lab winners!
TypeBlog Post...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe...Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This year with... -
Editor's Choice, October 2016
TypeBlog Post...party (see all of our wonderful costumes in the pictures below, yours truly is dressed as Unikitty from...Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, ... -
Grad School Advice Part 1: Picking a Lab and a Project
TypeBlog Post... 26:10 : What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog Post... are some good guidelines to follow in order to ensure that people can quickly and easily identify what...reading the name. For more information about plasmid features and tips you can use in the lab, check out more... -
What's New in CRISPR - Winter 2018
TypeBlog Post...compared to monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids... from this Addgene blog post Read the paper in Nature Methods Find the anti-CRISPR plasmids Tagging... -
Grad School Advice Part 2: Building Community
TypeBlog Post...here: https://www.youtube.com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog Post...therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, Koeris helped change Novophage's... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...Seeing a real lab!” And several of our visitors made sure to tell us what we already knew about our Shipping... guests, as did how much work is put into making sure scientists all over the world know that we can help... -
A Scientist's Guide to Ebola
TypeBlog Post..., along with links to the relevant scientific literature. -In light of the current Ebola crisis, Science... anyone interested in EBOV’s past, present, and future, don’t miss Carl Zimmer’s coverage on the subject... -
2022 and 2023: Reflections and Looking Forward
TypeBlog Post...present, but the real promise of Addgene lies in our future. As we near our 20th anniversary, we have many ...wealth of history to learn from, and a compelling future to plan in the changing landscape of science. We... -
Your Top Requested Plasmid in 2015!
TypeBlog Post...experiments; large amounts of virus are required to ensure that you get full library coverage in pooled library...and genome-wide libraries for CRISPR screening." Nature methods 11.8 (2014): 783-784. PubMed PMID: 25075903... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...simpler and maybe smaller Cas systems could exist in nature. To do this, they mined a database of microbial...Lucas B., et al. "Programmed DNA destruction by miniature CRISPR-Cas14 enzymes." Science 362.6416 (2018)... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post... RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA of interest...better understanding of the phenomenon soon. Rest assured, as the PUF protein design tools continue to evolve... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...started making regulations such as preventing manufacturers from dumping hazardous waste directly into the...levels. Disposables are an accepted part of lab culture, but with the right incentives (for example, by... -
Reflections and Looking Ahead
TypeBlog Post...sharing research materials — we're accelerating the future of science. Want to learn more about our latest...consensus platform for antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post... so be sure to check back often! To find more viral vectors available through Addgene, be sure to check... -
Editor's Choice, September 2016
TypeBlog Post...King soundtrack and writing this post, it is my pleasure to announce that we once again reached new heights... remember the hazards of that chemical in your obscure growth medium, these apps can help make your lab... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...pooled format at a more-typical scale of tissue culture while still offering the potential for true biological...and minimize off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...It could [be used to screen for] morphological features, migration, or survival in different contexts.... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post...focus wholly on the science I love. This immersive nature of academia often means that scientists-in-training... video call. If you do decide to use Skype, make sure to follow the rules of successful, remote conversations... -
Top 10 Open Science Developments of 2013
TypeBlog Post...agencies with over $100 million in annual R&D expenditures to support increased public access to the results... guided by aspirations to promote open science, ensure good stewardship of clinical trial data, serve ... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...based on how each one impacted cell viability (see figure). Most of the mutations were found to be “neutral... gene aberration drivers of pancreatic cancer." Nature communications 7 (2016): 10500. PubMed PMID: 26806015... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...testing multiple gRNAs targeted to the same locus to ensure their effects are specific to that locus. The data...spreadsheet for submitting your gRNA sequences here. Features of the validated gRNA table You can access the... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post... can’t find the molecular cloning guide you were sure was in one of those four white binders. Thirty minutes...) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on the spine. ...