We narrowed to 1,042 results for: URE

... mean a world of mental relaxation. And, I’m not sure I should put this in print, but I actually let my... my daughter skip school a couple of times. Be sure to add time with your family, for yourself and for...just “do science” – we are scientists. We have to figure out a way to keep being ourselves and to be happy...

...behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can move freely..., learning, and memory. We’ll cover these in a  future blog post, so stay tuned!  References Alpár, ...rapid and long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390...

...transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version of LY6A. ...to the central and peripheral nervous systems." Nature neuroscience 20.8 (2017): 1172. PubMed PMID: 28671695...for widespread gene transfer to the adult brain." Nature biotechnology 34.2 (2016): 204. PubMed PMID: 26829320...

... that lack cookie monster are sterile due to a failure of primary spermatocytes to enter meiosis. Testis...Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430. ...segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801. PubMed PMID: 9390559. Additional...

...and S. cerevisiae (Tuller et. al., 2010). Figure 1: An overview of peptide synthesis. A ribosome...as Hoogsten base pairs and wobble pairs.  Figure 2: (A) The codon wheel depicts the 64 codons that...PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon usage bias, and...

...The detectable signal from this reporter can be measured and quantified to determine the presence and quantity...lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK detection system...2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020...

..., the envelope gene, and a polyA tail.   Figure 1: Lentiviral production uses three plasmids: (...in Medical Virology (Li et a., 2018).   Figure 2: To pseudotype with the SARS-CoV-2 spike protein..., Bieniasz PD, Jäger U, Porter CD, Friedman T, McClure MO, Weiss RA (1997) Sensitization of rhabdo-, lenti...

...Addgene. In vitro screening for the best gRNA Figure 1: Validation of double strand break (DSB) mediated...were fusing, you may have several mosaic mice. To figure out what alleles are present in these mice, you...favourite mammalian model organisms in the near future.  Many thanks to our guest blogger Samantha Young...

...than the other (Sandomenico et al., 2020). Figure 1: Comparison between the IgG antibody and scFv...of panning and scFv characterization.    Figure 2: Phage display starts with a pooled library of...Bates A, Power CA (2019) David vs. Goliath: The Structure, Function, and Clinical Prospects of Antibody ...

... dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often leads...database, which relies on CAGE-seq to directly capture the mRNA cap, provides the most accurate mapping...examined the ability to use sequence-based and other features to nominate gRNAs that are likely to be active...

...pipeline processing (Nextflow), cloud infrastructure, and secure collaboration.  In that post, we talked...

...accompanied by new or refreshed figures made using BioRender.com. Figure 1: PRIME editing Please...

...the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian use. On the second...Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR...

... significantly away from the functionality and features that previously made the platform a mainstay of...not delete @addgene on X. This will additionally ensure that the @addgene handle is always associated with...

...interrogate many scientific questions. But these tiny creatures do tend to come with a rather astonishing ability...system for locating and containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd...

...sharing all the incredible guest posts and stories featured in our blog in 2023. Some of these were written...Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes...

...whether or not their achieving it. Chapter 5 - JEI's future - getting more involved in the classroom by building... lab for the first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing, Mark...

... by Xi Chen (@seleena78)   Honorable Mention: Culture Warrior The Moore lab, who was full of holiday ...honorable mention for their second submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire...

..., you will be able to search for your favorite features, such as serotype, promoter, or tool type. Drilling...accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, we hope to...

...guide page. For more protocols and information, be sure to check out the Trono Lab website. If you have ... plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours...

...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...@seleena78)   Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram...

...Fun stats and trivia begins! Tuesday: Depositor features (plasmid deposits and blog posts showcasing the...year in recent years. Despite COVID-19 and lab closures, we’re expecting to reach ~14,000 plasmids deposited...

...  Fig. 1: Meet...well, frankly, we're not sure yet!   This as-yet–unnamed friend is a huge ...mascot, an antibody joining us on our scientific adventures.  You can submit your name suggestions here or...

...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe...Christmas tree! It is that time of the year again: miniature #Christmas tree goes up at home!This year with...

... purity (260/280 nm and 260/230 nm ratios) and measure the concentration. Since this is a beta project...you’d like to see as part of the service in the future using the Cloning Grade DNA Suggestion Form.  ...

...party (see all of our wonderful costumes in the pictures below, yours truly is dressed as Unikitty from...Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research executive director, Gary McDowell, ...

... 26:10 : What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process...

... are some good guidelines to follow in order to ensure that people can quickly and easily identify what...reading the name. For more information about plasmid features and tips you can use in the lab, check out more...

...compared to monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids... from this Addgene blog post Read the paper in Nature Methods Find the anti-CRISPR plasmids Tagging...

...here: https://www.youtube.com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate... Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve the Research Process...