We narrowed to 838 results for: ANG

1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...monkeys. Surprisingly, we found that this small change made a big difference. Replacing the mCherry with...understanding of the physiology and pathology of the basal ganglia in movement disorders.   References and Resources...

2. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the Addgene... Angela Abitua...

3. Summer SciComm Series: Cognitive Load

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   ...otherwise it would be a lot quicker to pick up new languages!) Jargon has a place in science communication,...student groups and being aware of cognitive load changed the way I thought about engaging with learners....

4. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ...al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert et al., 2014)...: 24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human cells using...

5. The Future of Research Symposium Boston 2015

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   ...would be the effects, positive and negative, of changing the workforce structure? Helping to answer some...Roach. "Science PhD career preferences: levels, changes, and advisor encouragement." PloS one 7.5 (2012...

6. Plasmids 101: The Wide World of Natural Plasmids

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   ...that cause disease. While these plasmids can be dangerous, they also provide researchers with new means ..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan...

7. Making CRISPR Plasmids Using Fragmid

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   ...means that each component can be designed with an overhang specific to the type of module it is (Pol 2 protomer...for the Fragmid cuts, clone in the appropriate overhang for your module type, and voilà! You’ll have your...

8. Hot Plasmids February 2024

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   ...Adapted from Zhang et al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al....

9. Rabies and Neuronal Tracing

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   .... It wasn’t until later time points that longer-range connections were seen. This study addressed the ...Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics...

10. Adapting Toehold Switches to Detect Zika Virus

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    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources on Addgene.org Check out All...

11. The Materials Science of Optogenetics Experiments

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    ...excessively long that it becomes tangled. Furthermore, to give the animal the full range of motion, a commutator...

12. Degrading DNA with Cascade-Cas3

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    ... DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...

13. The AAVantages of AAV in CRISPR Screens

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    .... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler, D. J., Zhan, W., Gallagher, T. L.,...

14. Viral Vectors 101: Producing Your rAAV

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    ...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection...the density gradient material by dialysis/buffer exchange and further concentrate the virions if needed....

15. Plasmids 101: Degron Tags

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    ...of an exogenous small molecule). Degron tags can range in size from several kDas up to ~35 kDa and are ...10.1038/s41467-022-33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable...

16. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...same type will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor...interactions. Additionally, thanks to the modular arrangement of vectors included in tagging systems like SapTrap...

17. Hot Plasmids and Viral Preps - May 2021

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    ...infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface receptor....01.30.428974   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

18. The Scientific Conference Poster Session: Tips for Success

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    ...background relative to your research? Tailor your language to your audience so that they can digest what ... contact with the presenter after the session. Exchange business cards or jot down their name and affiliation...

19. Plasmids 101: Knockout/Knock-In Plasmids

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    ...stranded breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of ...and animal models, their efficiency is very low, ranging from undetectable to 0.1%. In contrast, new genome...

20. PITChing MMEJ as an Alternative Route for Gene Editing

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    ...targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ to insert FLAG... plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016...