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We narrowed to 835 results for: ANG

Showing: 541 - 560 of 835 results
  1. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...would be the effects, positive and negative, of changing the workforce structure? Helping to answer some...Roach. "Science PhD career preferences: levels, changes, and advisor encouragement." PloS one 7.5 (2012...
  2. Rabies and Neuronal Tracing

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    Blog Post
    .... It wasn’t until later time points that longer-range connections were seen. This study addressed the ...Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics...
  3. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ...that cause disease. While these plasmids can be dangerous, they also provide researchers with new means ..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan...
  4. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...means that each component can be designed with an overhang specific to the type of module it is (Pol 2 protomer...for the Fragmid cuts, clone in the appropriate overhang for your module type, and voilà! You’ll have your...
  5. Hot Plasmids February 2024

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    Blog Post
    ...Adapted from Zhang et al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al....
  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources on Addgene.org Check out All...
  7. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...stranded breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of ...and animal models, their efficiency is very low, ranging from undetectable to 0.1%. In contrast, new genome...
  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...background relative to your research? Tailor your language to your audience so that they can digest what ... contact with the presenter after the session. Exchange business cards or jot down their name and affiliation...
  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...
  10. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    .... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler, D. J., Zhan, W., Gallagher, T. L.,...
  11. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...of an exogenous small molecule). Degron tags can range in size from several kDa up to ~35 kDa and are typically.../s41467-022-33246-4  Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable...
  12. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection...the density gradient material by dialysis/buffer exchange and further concentrate the virions if needed....
  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface receptor....01.30.428974   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...
  14. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...same type will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor...interactions. Additionally, thanks to the modular arrangement of vectors included in tagging systems like SapTrap...
  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ to insert FLAG... plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016...
  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post
    ... and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while technically... 29668265. PubMed Central PMCID: PMC6002863. 4. Liang, Zhen, et al. "Efficient DNA-free genome editing...
  17. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...figure 8 in Chan et al).  References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered AAVs for efficient...
  18. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...factor IGF2 open reading frame to relax imprinting (Zhang et al., 2017). This upregulates expression of IGF2...PMID: 23452860. PubMed Central PMCID: PMC3664290.  Zhang, Yiqun, et al. "CRISPR Cas9-guided chromatin immunoprecipitation...
Showing: 541 - 560 of 835 results