We narrowed to 843 results for: ANG
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TypeBlog Post...otherwise it would be a lot quicker to pick up new languages!) Jargon has a place in science communication,...student groups and being aware of cognitive load changed the way I thought about engaging with learners....
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Transferable Skills Guide: Problem Solving
TypeBlog Post...protocol modifications, and ultimately decided on the changes to implement. As with any skill, there will always...solving skills and learn to apply them flexibly in a range of contexts. As such, scientists are uniquely prepared... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...monkeys. Surprisingly, we found that this small change made a big difference. Replacing the mCherry with...understanding of the physiology and pathology of the basal ganglia in movement disorders. References and Resources... -
Click Biology: How to Click and Play to Explore Biology
TypeBlog Post...parts in the micromolar or nanomolar concentration range. Reactions to happen under lots of conditions ...from Promega, including many HaloTag plasmids Exchangeable HaloTag Ligands HaloTag Reversible binding of... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the Addgene... Angela Abitua... -
Antibodies 101: Fab Fragments
TypeBlog Post.... Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain on a Fab’...:703–706. https://doi.org/10.1177/34.6.3084626 Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post...abilities is that its fluorescence emission spectra changes when it goes from a more neutral pH compartment...emission spectra. Component Excitation Emission pH Range DsRed.T3 488, 543*, 568 nm 587 nm ~4.9 - ... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert et al., 2014)...: 24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human cells using... -
The Future of Research Symposium Boston 2015
TypeBlog Post...would be the effects, positive and negative, of changing the workforce structure? Helping to answer some...Roach. "Science PhD career preferences: levels, changes, and advisor encouragement." PloS one 7.5 (2012... -
Hot Plasmids February 2024
TypeBlog Post...Adapted from Zhang et al. 2023 with permission. Find Cas-PAGE plasmids here! Zhang, Z., et al.... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...that cause disease. While these plasmids can be dangerous, they also provide researchers with new means ..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...means that each component can be designed with an overhang specific to the type of module it is (Pol 2 protomer...for the Fragmid cuts, clone in the appropriate overhang for your module type, and voilà! You’ll have your... -
Rabies and Neuronal Tracing
TypeBlog Post.... It wasn’t until later time points that longer-range connections were seen. This study addressed the ...Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources on Addgene.org Check out All... -
The Materials Science of Optogenetics Experiments
TypeBlog Post...excessively long that it becomes tangled. Furthermore, to give the animal the full range of motion, a commutator... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...background relative to your research? Tailor your language to your audience so that they can digest what ... contact with the presenter after the session. Exchange business cards or jot down their name and affiliation... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface receptor....01.30.428974 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids... -
Degrading DNA with Cascade-Cas3
TypeBlog Post... DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post.... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler, D. J., Zhan, W., Gallagher, T. L.,... -
Plasmids 101: Degron Tags
TypeBlog Post...of an exogenous small molecule). Degron tags can range in size from several kDas up to ~35 kDa and are .../s41467-022-33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable...