...Hot Spring at YNP, and since then, many researchers have searched for novel organisms and enzymes from these...our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...

...Ford  Listen to the riboglow podcast segment Researchers from the Palmer lab at the University of Colorado...cleavage activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast segment The...reporter plasmid! Martin et al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ...

...requirements. To solve this problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo...efficiency. Higher sensitivity to off-targets More research is needed to compare CRISPR and NgAgo, but it ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

... red light instead of blue, which will allow researchers to independently control the activity of two .... Understanding the brain through optogenetics research Addgene: What are some of the big problems that...Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab Sign ...

...Inferior: How Science Got Women Wrong - and the New Research That's Rewriting the Story by Angela Saini "This...in what happens when scientists bring biases to research and don't collaborate with others to bring diverse... series, there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...

...), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles have some advantages...isolation protocol by Maguire and colleagues, other researchers have tried to modify it to improve yields. For... viral protocols and videos Find AAVs for your research ...

... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...systems for mammalian screens, which enables researchers to maximize the chance of transducing a only ...downstream of the apoB gene. Using this reporter, the researchers explore many aspects of ApoB-LP biology, including...these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as the eFRET sensors, ...

...by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver.  Most types of biological...enough to engineer it to suit our purposes? In search of simple biological machines R bodies can repeatedly...environment.   Jessica Polka is a Postdoctoral Research Fellow with Pamela Silver in the department of...

...around 100-fold. These improvements will allow researchers to better fine-tune the level of output gene ... Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA. Nature. 2005 Nov 24;438(7067):441...Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Cell. 2009 Jun 26;...

...undoubtedly constitute a valuable tool for the research community. You can find the AAV vectors for infecting...imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin and Ryohei Yasuda ...sodium/potassium pump ATP1A1 in addition to a researcher’s locus of interest. NHEJ- or HDR-induced mutations...Cre recombination. Each knockout includes unique barcodes and universal priming sites to ensure the collection...

...the University of Alberta. If you’re like many researchers these days, you are ready to take (if you haven...are often impractical for small projects. Most researchers instead turn to so-called “mismatch nuclease”...knockout of HIV co-receptor CCR5. Nucleic Acids Research 43, 5560–5571 (2015). PubMed PMID: 25964300. PubMed...

...mutational abilities of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell...flexibility to address many different types of research questions. Are you ready to start EvolvR-ing your...Perez-Pinera. "The continuously evolving CRISPR barcoding toolbox." Genome biology 19.1 (2018): 143. PubMed...

...γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus... 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or drive high...Cycle Popular Retroviral Vectors and Their Use in Research More resources on addgene.org Addgene Protocols...

...base editors for your research Read our CRISPR guide Find plasmids for your research ...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a...

...GPCRs. This collection, called TRUPATH, allows researchers to identify specific G proteins activated by ...s still a lot to learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic...chemogenetics guide Find chemogenetics plasmids for your research ...

...-level positions right out of college. Prior to March 2020, internships were a fiercely competitive endeavor...applying to several opportunities. In an age of scarcity, the rational human being will tend to clinch ...out other tools and resources for early career researchers Applying for jobs? Check out these blog posts...

...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular biology and...inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM can help...Perfect Plasmid Backbone Find Plasmids for Your Research ...

...engineer and scientist, I like to envision that my research will have a direct impact on healthcare and the...result, I have occasionally pondered translating my research into a startup company. However, like many like-minded...incorporated into Novophage Therapeutics, Inc. in March 2009 and tested in the “market” by competing in ...

...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...low resolution and can’t be used in vivo. Some researchers have used fluorescently tagged DNA-binding proteins...CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single genomic locus,...