We narrowed to 648 results for: ATO
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TypeBlog Post...technologies. Message or follow him on twitter @Vopinator. References: Halpin-Healy TS, Klompe SE, Sternberg...
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Viral Vectors 101: The Retroviral Lifecycle
TypeBlog Post... Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...level of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post... in Parkinson’s disease. Knowing the primary investigator where the plasmid was created can narrow your... -
MXS Chaining
TypeBlog Post...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique... -
Plasmids 101: Protein tags
TypeBlog Post...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post... the proper cell line is used; several common laboratory cell lines including 293 were immortalized with... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves... eat, drink, chew gum, or apply makeup in the laboratory. Before you start your experiment, make sure ...Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some times...officer. BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be followed ...not be worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names...names and contact information of the laboratory manager should be clearly visible in the room and on the ...vaccination or titer prior to starting work in the laboratory. For some biohazardous waste, an autoclave or... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...an organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson... -
Bioinformatics at Addgene
TypeBlog Post...much information as possible from depositing laboratories and associated publications, but our quality... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution... -
Transferable Skills Guide: Creativity
TypeBlog Post...This is why perfectionists tend to also be procrastinators. If you fall into this category, get out of... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...antibiotic works. Another example is Zeocin, a DNA intercalator, which affects all kingdoms of life. Of course... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...seeing our resources shared via social media, on collaborators’ websites, or as a recommendation in a Reddit... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...