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  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...marker help synthesize? Counterselection? (growth-based positive selection for the loss of the marker gene...

  4. Lentivirus Production

    Type
    Protocol
    ...solution will drift rapidly upon addition of acid or base. Add only a few drops at a time, allow them to mix...

  6. Retrograde AAV viral preps

    Type
    Collection
    ...Available Retrograde AAV See our retrograde AAV based on the functional catagories listed below. Narrow...

  9. RNA Extraction Without A Kit

    Type
    Blog Post
    ...and RNAProtect® from Qiagen, are ammonium sulfate-based solutions that work by inhibiting RNase activity...

  14. Addgene: The First Twenty Years

    Type
    Blog Post
    ...provide recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill...

  17. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...beyond generating frameshift mutations or single base pair edits, these enzymes are worth taking a look...Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is thus not helpful...
Showing: 541 - 560 of 705 results