CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
Type
Blog Post
...meets two conditions:
1) The sequence is unique compared to the rest of the genome.
2) The target is present...found. If you’re lucky enough to find a SpCas9 compatible PAM at your site of interest, that is the most...expanding number of Cas9s with looser PAM requirements compared to SpCas9. xCas9 has a PAM requirement of NG, ... proteins. The Synergistic Activation Mediator complex, generally the strongest activator, consists of...