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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 90 results for: Comp;

Showing: 1 - 20 of 90 results

  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...major histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex, class...major histocompatibility complex, class II, DO beta DOB HLA-DPA1 major histocompatibility complex, class... Name Synonyms C3 complement component 3 ARMD9, ASP, CPAMD1 C5 complement component 5 CPAMD4, FLJ17816...R33729_1, SF20 C3 complement component 3 ARMD9, ASP, CPAMD1 C3AR1 complement component 3a receptor 1 AZ3B...HNFAG09 C5 complement component 5 CPAMD4, FLJ17816, FLJ17822, MGC142298 C5AR1 complement component 5a receptor...major histocompatibility complex, class II, DM beta D6S221E, RING7 HLA-DOA major histocompatibility complex...

  2. Antibody Guide

    Type
    Collection
    ...capture protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced...is licensed to multiple companies for sale. Before purchasing, directly compare the product information... size comparison of peptides. Independent antibody validation This method validates by comparing your ...configuration of a protein or a protein:protein complex. Due to their increased manufacturing requirements...from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv -...nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative affinity reagents (left to right...conjugating and signaling molecules, as some buffer components can interfere with the conjugate or conjugation...

  3. CRISPR Guide

    Type
    Collection
    ...type I Cas effector, composed of several separate Cas enzymes that form a complex called Cascade (see ...transposase components. Type I (generally) includes tnsA , tnsB , tnsC , and tniQ . In comparison, type V-...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became...mechanism Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated...Cas enzyme). The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding...have extended CRISPR applications to increasingly complex functions, including the ability to selectively...mechanisms of CRISPR and some of the ways the CRISPR components are leveraged to achieve specific goals, such...

  4. Plan Your Experiment

    Type
    Collection
    ... process of interest. Do you want to: Generate complete and permanent loss of gene expression or function...you are ready to start designing the specific components (Cas enzyme and guide RNA) for your experiment...genetic manipulations require different CRISPR components. Selecting the genetic manipulation you plan ...plan to perform will help you narrow down which components you’ll need for a given experiment. In the table... will cover strategies for designing the gRNA component for each category. Genetic Manipulation Application...Requires a repair template Lower editing efficiency compared to NHEJ knockout Cut Nick Base Edit Generate specific...perfect world, your gRNA sequence would be an exact complement to your target sequence with no homologous sites...

  5. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...nuclease form a complex with each individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out ...a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy...out the DNA sequence complementary to the crRNA. In Type II CRISPR systems, a potential target sequence ...after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target...strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems...classes based on the structure of the effector complex that cleaves the genomic DNA. Type II CRISPR was...following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain effector) Type I ...

  6. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Determine the reverse complement of each guide sequence. Example reverse complement sequences of the Pim1...repair. Deletions have potential advantages as compared to single-site small indels given the efficiency...oligos for each guide and its associated reverse complement including additional nucleotides for cloning ...sequence and “AAAC” before the guide’s reverse complement for cloning into the pX330 vector using BbsI ...sequence. Add a C at the 3’ end of the reverse complement oligo ( e.g., sgRNA-A in Table 4 ). The resultant...not add C to the final position of the reverse complement oligo. In this case, the resultant oligos would...μl reaction mix for each guide and its reverse complement: 1.0 μl sgRNA 24- or 25-mer oligo (100 μM; see...

  7. mTOR Pathway

    Type
    Collection
    ... kinase, is found in two complexes, mTORC1 and mTORC2. Each complex contains a unique subunit...tensin homolog Rictor RPTOR independent companion of MTOR, complex 2 SGK Serum/glucocorticoid regulated ... to find available plasmids for the gene. For components with multiple isoforms or subunits, individual...links to each gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation... RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras homolog enriched in brain S6K Also known...Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...sociated c omplex for a ntiviral de fense) complex, comprised of a combination of Cas5, Cas6, Cas7, Cas8...to the difficulties in getting all components of the Cascade complex to express equally. To combat this...system that can cause large genomic deletions. Compared to deletions generated by Cas9, large deletions...nature, Class 1 systems are largely underutilized compared to their Class 2 counterparts like Cas9. This ...though many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism...

  9. Promega Plasmid Collection

    Type
    Collection
    ... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)... of a chemical compound and its target protein. The apparent affinity of test compounds is measured by...help advance science while reducing operational complexity. This unique collection contains plasmids developed...kDa) subunit and a small complementary peptide, Small BiT (SmBiT; 11 amino acids). This system is ideal ...by competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion protein...

  10. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...instead recruiting the transposase (comprised of multiple components) to target locations in the genome...type I Cas effector, composed of several separate Cas enzymes that form a complex called Cascade ( C RISPR...for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs...V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type I, type V-K...

  11. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Biolabs website. For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...allow for dual labeling and visualization of complementary protein function or localization. ACP and MCP...cell surface proteins with chemically diverse compounds. J Am Chem Soc. 126(29):8896-7. PMID:15264811 ...

  12. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...designed to specifically localize to cellular compartments or organelles. Addgene’s database includes many...imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas...GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector...;101(50):17404-9. Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol...sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive Dual- and Triple-Feature Intersectional Single-Vector...locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded sensor and small molecule...NAD/NADH ratio changes in different cellular compartments. Biochim Biophys Acta. 2013 Nov 25. pii: S0304...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Yeast pGBKCg - Gateway compatible Y2H vector LEU2 Yeast pGADT7-GW - Gateway compatible Y2H vector - AD pGADCg...pGADCg - Gateway compatible Y2H vector BASTA Plant pGTQL1211YN - Gateway compatible BiFC vector (pEarleyGate201... proteins, and more pGBKT7-GW - Gateway compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA...Gentamicin Varies pJL-TRBO - An improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression...

  14. Cre-lox system

    Type
    Collection
    ...recombination has occurred, allowing for direct comparison of Cre+ and Cre- cells. While Cre-lox recombination...Mammalian Pelczar 51267 pCAG-Co-InCreN N-terminal component of the Co-InCre system CAG Mammalian Pelczar 51268... 51268 pCAG-Co-InCreC C-terminal component of the Co-InCre system CAG Mammalian Pelczar 51273 pCAG-Roxed-Cre...Eisenhoffer 131782 pME-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry ...Eisenhoffer 131785 pAAV-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer...Eisenhoffer 131786 pLenti-CreLite CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector...Birchler 140131 pAAV-EF1a-NCreV N-terminal Cre component split with Vivid photodimers EF-1 alpha AAV Zeng...

  15. Distribution to Industry

    Type
    Collection
    ...High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using comprehensive end-...Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement (MTA), including...Scientist Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using...using the materials in the order. After the RSA is complete, Addgene will send you a copy of the MTA, which...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...most common is to use fluorescence microscopy to compare the localization of your protein of interest with...Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein Collection...table below to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ...Firefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...Fifrefly luciferase fragment for split-luciferase complementation assay in plants using Gateway cloning. Use ...-RLuc Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328...deep-learning approaches. Split Luciferase Complementation assays can report on protein-protein interactions... : Constructs for split firefly luciferase complementation assays in plants. Bioluminescence Resonance...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...Activation Mediator (SAM) is an engineered protein complex for the transcriptional activation of endogenous...endogenous genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating... : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with...vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker 61427 : lentiviral...transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J...

  19. Bacterial Expression Systems

    Type
    Collection
    ...collection of Golden Gate compatible vectors for bimolecular fluorescence complementation experiments in plants... a library of promoters and purification tags compatible with Golden Gate modular cloning (MoClo) for ...transfer (FRET) and bimolecular fluorescence complementation (BiFC) are techniques that have been widely...Introduction to FRET Bimolecular Fluorescence Complementation Makes Visualizing Protein–Protein Interactions...

  20. Tetracycline Inducible Expression

    Type
    Collection
    ...Tet-Off and Tet-On. Read on to learn more about the components of tet systems and how to use them, or skip ahead... Systems Subsequent efforts have improved the components of these systems for better efficiency, sensitivity... transfected or transduced cells express both components in the same relative ratio. Different generations...transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter, and...et al., 2016 (Link opens in a new window) for comparison of Tet-On systems in different applications. ...
Showing: 1 - 20 of 90 results