We narrowed to 660 results for: EPO
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TypeBlog Post... the Patron Lab. As with all plasmids in the repository, we highly recommend reading the associated publications...
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Site Directed Mutagenesis by PCR
TypeBlog Post...regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional analysis of proteins... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...co-expression of a suppressor tRNA (SupD) instead of the SepOTSl can encode a non-phosphorylated serine at the TAG... -
Evolution of Lab Techniques
TypeBlog Post...already commercially available although they are reported to have high error rates (10). It is projected... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...drop down menu of common cloning options in the deposit process in 2014 because of its gain in popularity... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog Post...editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...(the current median postdoc starting salary as reported by Paula Stephan is $40,000) Increase Knowledge... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...ten colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct... -
Lentiviral Prep Service
TypeCollection... made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...on each item's material page. Actual titers are reported with each shipment. For more details about our... that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting specific...of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...enter the cell with the concentration gradient to depolarize it (Nagel et al., 2003). Inhibitory opsins are... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post... Interest Into a Variety of Expression Vectors Deposit Your Microbiology Plasmids ... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed... -
TALEN Plasmids and Kits
TypeCollection...repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX...with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition... plasmids. Return to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into...visualize which cells have undergone recombination. Reporter plasmids can also be useful for lineage tracing... -
Important Considerations When Using AAVs
TypeBlog Post... complexes to study neural function. Often the reporter proteins associated with these complexes are so... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...Addgene blog All Antibody Topics Antibody Data Repositories and Search Engines Antibodies 101: Producing... -
Finding Your Science Policy Path
TypeBlog Post...science policy skills in graduate school via this report from the Scientific Citizenship Initiative. ... -
Antibodies 101: Flow Cytometry
TypeBlog Post...tag your protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside...