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We narrowed to 802 results for: Eng

Showing: 541 - 560 of 802 results

  1. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...entire time talking to the poster, as opposed to engaging with you? Be sure to use your poster as a visual...they are following along. This will not only re-engage you with those who sought out your poster, but ...

  3. Transferable Skills: Negotiation

    Type
    Blog Post
    ...natural. Creative negotiation skills will help you strengthen relationships with your colleagues and supervisors...help you get more of what you want and can also strengthen your relationships with others who know that ...

  4. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...identified in nature, while many more have been engineered in labs. This vastly increases the possibilities...S. E., Muller, A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, ...

  5. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production Tips for Titering Your...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

  7. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...mouse knockout lines should increase. New genome engineering tools also offer hope for the creation of new...models in species that were previously difficult to engineer, like rats. If you have tools for creating knockout...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh ...) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated this system...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...scientists of all types to stay up to date and engaged with new advances in published science. They also...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    ...). Additional resources on the Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24...pSiM24 for simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org...

  13. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...from very high cell density suspension. Biotechnol Bioeng. 2021 Sep;118(9):3499-3510. doi: 10.1002/bit.27776...Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock in vials causes...

  15. An Introduction to Adenovirus

    Type
    Blog Post
    ...viruses, with a linear genome between 26–45 kb in length. Human adenovirus type 5 (hAdV-C5) can be considered... like other viral vectors, though significant challenges remain (Wold & Toth, 2013).  While the pre-existing...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of...2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...Cpf1-mediated cleavage are 42-44 nucleotides in length, about the same size as Cas9’s, but with the direct...Figure from Kleinstiver et al., 2016. Cpf1’s strength may lie in its low off-target editing rates, determined...
Showing: 541 - 560 of 802 results