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We narrowed to 1,141 results for: Gene

Showing: 541 - 560 of 1141 results

  1. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...her current research, Sheen aims to identify new genes related to PZA-resistance by using different approaches...as pACBSR-hyg and pFLP-hyg will be used to create gene deletions of important unknown regulatory regions...growth will then be measured to link the deleted genes to specific functions in biofilm formation. The...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...

  2. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...for the generation of one or more mutations – it is as easy as obtaining a plasmid with the gene of interest...interest (search Addgene plasmids by gene here), designing primers with your desired mutation, performing... desired promoter, selectable marker, and target gene, for example, are amplified with overlapping regions... E.coli for plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity...multiple mutations in one or multiple fragments, and generate constructs for producing single-guide RNAs – but...the NEBuilder HiFi DNA Assembly Mix include:  Generation of constructs with multiple point mutations Cloning...sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple point mutations NEBuilder...

  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ... nucleic acid sequence of the antibody-encoding genes over time as the hybridoma cells divide. These changes...before culturing or they can clone the genes from the hybridoma into a plasmid to create a recombinant...Figure 2: Changes to the heavy chain or light chain genes within a hybridoma impacts the specificity, avidity...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...above, genetic drift is one concern when using hybridomas to produce monoclonal antibodies. Genetic drift...the target molecule. Although it’s possible to generate antibodies against many types of molecules including...recognize proteins. Figure 1: Steps in the generation of hybridomas for monoclonal antibody production...

  4. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the ACE ...Wanisch  Listen to the optogenetics podcast segment LOVTRAP is an optogenetic system that was recently... the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where...function is more specific and much faster than any genetic or pharmacological manipulations on cells, and ... riboswitches used in the Riboglow platform at Addgene. Braselmann E, et al. Nature Chemical Biology...

  5. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ... we can make Addgene better and make scientists’ lives easier. Why transition to Addgene? So why did ...more satisfying, because Addgene is a non-profit, we can focus more on generating blog content that scientists... that PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about ...Harvard University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days... less time wrangling bacteria and instead help Addgene fulfill its mission of helping scientists share...did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...didn’t, it would have been unwise of me to join Addgene. This is particularly true in my position as an...

  6. Typing CRISPR Systems

    Type
    Blog Post
    ...domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2...sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental ...straightforward as it seems. There is not a single gene that is shared across all CRISPR types; the fast...together because they contain a distinct Cas7-type gene. The exact mechanism of type IV systems is not known... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...the array of CRISPR technologies. Learning the general principles of this classification system will help...A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A. Cas9 was the first to be adapted...

  7. Synthetic Biology - Algal

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    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in algae. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  8. Synthetic Biology - Bacterial

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in bacteria. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  9. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication...A list of Addgene plasmids for use in synthetic biology applications, or from synthetic biology labs....to see added to this list, please email help@addgene.org . Synthetic Biology Plasmids Search the table...

  10. Synthetic Biology - Fungal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in fungus. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  11. Synthetic Biology - Plant

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in plants. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  12. Synthetic Biology - Yeast

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in yeast. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  13. AAV Packaged on Request

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    Collection
    ...Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian... more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources Viral Vector...recommended for research-grade use. Many chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of: serotype (AAV1, AAV2...Large 50 × 100 µL aliquots 5 mL *AAV2 yields are generally lower, with a minimum titer of 2 × 10 12 GC/mL...production Density centrifugation purification Addgene’s comprehensive quality control process: Titering...

  14. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...’s first genetic engineering company, Genentech, was founded.  This commercialization of gene editing ...before the discovery of genes themselves.  How has scientific progress in genetic editing impacted the world... DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition sites had...followed by non-homologous end-joining that results in gene knockouts.   Star Trek: Into Darkness (2013) which...The major drawback of all the endonuclease-based gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas...Gregor Mendel on the genetic inheritance (1866) and just when the Mendelian genetics was being revived by...Zinc-Finger Nucleases. Genetics 188:773–782 . https://doi.org/10.1534/genetics.111.131433 Doudna JA, Charpentier...

  15. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...URL “http://n2t.net/addgene:87155” where the Addgene registered prefix and the Addgene ID # are directly...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” So when citing...Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite plasmid...through Addgene and find verified information about each plasmid item. Given a specific Addgene ID # listed...available through Addgene. Let’s take a look at lenti-Cas9-VQR-Blast, which has the Addgene ID #87155. The... as “RRID:Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will...contain the Addgene ID # and the information necessary to indicate that this ID is managed by Addgene. How to...

  16. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...when trying to edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient...formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting in the formation ...always positioned correctly to target a particular gene. Furthermore, a target sequence may have high homology...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog  Read more about Cpf1...sequences While PAM sequences for the commonly used S. pyogenes Cas9 (3'-NGG) are abundant throughout the human...circumvent this limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences, 2) the...Cas9 homologs derived from species other than S. pyogenes, and 3) the use of non-Cas9 enzymes. (For more...

  17. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter system to identify... (Katti et al., 2020). They named this reporter “Gene On” or GO. GO works by affecting protein translation...fluorescent proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter...and enrich for base-editing events in vivo. TLDR: Gene On (GO) from the Dow lab is a flexible reporter ...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission...hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that contains an ACG start...

  18. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... maintain the genes they carry among organisms belonging to many different phylogenetic groups (Jain and...that the plasmid is conjugative and carries the genes necessary for transfer initiation between bacterial...plasmids for DNA cloning in Gram-negative bacteria. Gene, 70(1), 191–197. https://doi.org/10.1016/0378-1119... S. (2011). Broad-host-range plasmid vectors for gene expression in bacteria. Methods in Molecular Biology...viscosus, Aerobacter aerogenes, Aeromonas hydrophila, Agrobacterium tumefaciens, Alcaligenes eutrophus, Azotobacter...salmonicida, Agrobacterium tumefaciens, A. rhizogenes, Alcaligenes eutrophus, Enterobacter sp., Erwinia amylovora... broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org/10.1007...

  19. Synthetic Biology - Mammalian

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in mammalian systems. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Synthetic Biology - Worm

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you have suggestions...Browse Addgene's collection of synthetic biology plasmids for use in C. elegans. Plasmid...Standards SynBio Blog Genome Engineering Browse Addgene's collection of synthetic biology plasmids for use...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 541 - 560 of 1141 results