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We narrowed to 911 results for: IRE

Showing: 541 - 560 of 911 results

  1. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...guest blogger Melanie Fox, founder and executive director of Central Indiana Science Outreach and a Postdoc...as a student. They didn’t always know the best direction to guide me because they had no experience with...Melanie Fox is currently the founder and executive director of Central Indiana Science Outreach and a Postdoc...

  2. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...sgRNAs and Cas9 are required for any application. If you want to include a homology directed repair (HDR) template...straightforward. Likewise for step 4, as this ties directly back to your specific goal. When thinking about...

  3. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...big science,” a massive, expensive project that required collaboration from scientists around the world... undermining science are even more immediate and dire than Zimmer could have imagined. Does this detract...about CRISPR alone, and Zimmer himself has written entire books about optogenetics. Defining science and ...

  4. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...to ddPCR. Droplet digital PCR (ddPCR) does not require a standard or a reference, meaning saving on reagents...20,000 droplets. Each droplet contains the material required for a mini amplification reaction to take place...for PCR experiments.  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet...

  5. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...dCas9, require Zinc-finger or TAL proteins targeting a specific genomic locus, which can require multiple...genomic region was an intensive process. One method required insertion of a recognition sequence into the genomic...

  6. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...mammalian antibiotic selection gene. However, it does require a slower transformation recovery period than amp...zeocin resistance by expressing a protein that directly binds to zeocin, preventing it from binding DNA...low rate, there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    ...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...response to adenoviral vector infected cells does not require de novo viral gene expression: Implications for...1996). The adenovirus death protein (E3-11.6K) is required at very late stages of infection for efficient...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...either briefly boiling the sample before use or by directly adding a small volume of the sample to the PCR...tell you whether the insert has the correct directionality. Verifying the insert sequence with Sanger ...

  9. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...from long written proposal narratives. Now they require grant seekers to use online applications with significant...to and proof of compliance with the grantor’s requirements as well as state and federal regulations. A ... worked for an organization in a position that required some involvement in the grants process and managing...

  10. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...of the edited sample. You can also download the entire analysis as a ZIP file by clicking the Download...the inferred distribution of indel sizes in the entire edited population of genomes. Hovering over each...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    .... Cre-dependent expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse an attractive...neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly demonstrated...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... sites. In cases like these, antibodies can be directly conjugated to agarose or magnetic beads with commercially... commercially available chemical agents. Directly conjugating the primary antibody has the additional ... particularly advantageous because they do not require centrifugation between washes which can cause loss...

  14. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...on pEcgRNA plasmid as only two 24 nt oligos are required and the BsaI linearized pEcgRNA backbone can be... in large amounts and frozen for future use or directly ligated with the annealed 24 nt oligos to generate...the above systems are great for experiments that require only single chromosomal integration (ex: genome...

  15. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral production...efficiency can vary across cell types and generally requires optimization of many variables, such as seeding...Using pooled libraries can be intimidating and require a large amount of experimental work, but with this...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...exposed even one time. Doctors and researchers don’t entirely understand why some people develop the disease...one of the best ways to improve the traditionally dire prognosis for mesothelioma patients is to catch ...inhibitor) prevents the growth of new blood vessels required to sustain growing tumors. Studies have shown ...

  17. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything..., work-life negotiation, and mentorship. We can direct people to the AMA in the future and link to it ...an organizer/promoter was that I didn’t have the direct link to send people until the day of the AMA. This...

  18. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...recombinase recognizes FRT sites and initiates site directed gene recombination between those sites. FRT sites...with low concentrations of vectors expressing the required recombinase. The high concentration of FLEx vectors...PMC41457. 8. Schnütgen, Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination...

  19. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...all the steps and technical considerations are directly applicable to the rat [6]. Technical differences...Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins." Nature...Central PMCID: PMC3082132. 9.Del Bene, Filippo, and Claire Wyart. "Optogenetics: a new enlightenment age for...

  20. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...which is ideal for observing and manipulating the entire developmental cycle. A majority of studies involving...most significant advances is the sequencing of the entire axolotl genome, revealing an abundance of genes...year 2000, becoming the first plant to have its entire genome sequenced (Koornneff and Meinke, 2010). ...
Showing: 541 - 560 of 911 results