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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,018 results for: LEA

Showing: 541 - 560 of 1018 results

  1. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...numerous discoveries have been made using the Cas9 nuclease in the absence of genomic alterations. These studies...sequence via a targeting gRNA. But dCas9 does not cleave the DNA. Much of the research using dCas9 has focused...research conducted in the Hodaka Fujii Laboratory, please also see our interview with Dr. Fujii. References..., et al. "A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." Science337.6096...

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...finding how-to videos on YouTube long before he learned (somewhat successfully) how to text or use Facebook... communication that you enjoy and that plays at least slightly to your strengths. Otherwise, you’re going...cost or freeware alternatives and how steep of a learning curve it will be to get to the necessary level...balance out cons. Flexible. Can allow people to learn in multiple ways from the same experience.  Can ...

  3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...research interests include proteomics, machine learning, and visualization for big biology. GPS for the...are critical? One hypothesis: the enhancers! This leads us to a three-step approach for designing an experiment...database. Aneesh's research interests span machine learning, proteomics, and user interfaces for math.     ...Catch Up on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome...

  4. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...and controls (see Tips below), and by validating leads with other methods. More common are false negatives...levels of bait and prey proteins - overexpression can lead to false interactions. Lowered expression levels...techniques! Minimizing false negatives: Interactions leading to detectable reporter signals depend on a number...problems with one or more of these parameters can lead to a high number of false negatives in Y2H screens...

  5. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...a cooler. Chances are one of your colleagues has a frisbee at least. Use it! 8. Organize If you’re doing...make an effort to build relationships with their colleagues. It can be easy to get caught up in the minutia...true. If your event requires a lot of set up or cleanup, ask for volunteers to help out. Delegate- it will...

  6. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ..., and they stepped up to apply for a grant which lead to their new career path.  A part of the career ...story for most established grant professionals is learning to succeed in grants through trial and error, ...increased their demand for stronger budgets with clear budget narratives, logic models, established partnerships... Throughout these moves and program changes, I learned to write grants for health, housing, economic development...

  7. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ... Make it clear that you are interested in the specific position you are applying to. Be clean and professional...letter, and if possible, send it to a mentor or colleague for a second look and helpful feedback!  What ...     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network Become A Good...

  8. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... Flow data is messy, so learning how to gate takes a lot longer than learning how to read a gating strategy...visualization tool, a quadrant will often be too clean for real-world data. Most plots will have a shape...    Of course, there are many more things to learn about when diving into flow cytometry data. There...

  9. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...Cas13, the "collateral" activity of the enzyme can lead to off targets and result in significant, negative...processing pre-CRISPR RNA into mature CRISPR RNA and cleaving RNA at positions defined by the target:spacer ...limited to small deletions and small insertions, leaving a several kilobase-sized gap in the gene editing...Abudayyeh. Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases bioRxiv 2021. ...

  10. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...researchers recognize their MANY transferable skills learned while working in the lab and offering advice on...think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable skill...get distracted by an email or sidetracked by a colleague. To innovate, you’ll have to focus. Find yourself...(2019). Embrace your weird face your fears and unleash creativity. Gallery Books. Boyes, A. (2019, November...

  11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... proximity of the fluorophore and subsequently leading to a positive signal. The same effect can be achieved...this approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (...assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound malachite...Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with...

  12. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...and ssDNA cleavage by canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Research...

  13. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... around, the plasmids are randomly positioned, leading to plasmid loss (Diaz et al., 2015).  Figure...designed small, high copy incompatible plasmids that lead to asymmetrical plasmid loss of the usually large...Addgene blog Read all of our Plasmids 101 blog posts Learn more about the origin of replication Find blog posts... Leah Schwiesow...

  15. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...also reversible: once the environmental trigger leaves, gene expression is no longer activated. However...N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the different types of Cas9 activators....3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next...More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on ...

  16. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...small RNA expression shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, ...lacI or lacIq). Can be induced by IPTG or lactose. Leaky promoter with somewhat weak expression. lacIq mutation...the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene...

  17. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...intuitive, qualitative interpretation of this gel is misleading. We can see why on the S-shaped curve. On the...on the band intensity axis. This effect causes misleading qualitative conclusions, and it’s also problematic...approaches Cartoons are nice and all, but we’ll leave you with this real-world comparison of different...of western blot normalization. May your blots be clear and your detection range be linear! Resources and...

  18. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...Optogenetics Guide to learn more about how these modules work, and read on to learn how they are put to ...factors (GEFs) activate their partner Rho GTPase, leading to behaviors like lamellipodia or filopodia formation...these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised a set of optogenetic...

  19. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Addgene blog Learn about research applications of antibodies in Introduction to Antibodies Learn more about...visualization methods. Stay tuned for a future blog post to learn more about these techniques!  Direct vs. indirect...what sort of protocol you will need to use. (To learn more about antibody validation, this commentary ...

  20. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ... the nonhomologous end-joining (NHEJ) pathway, leading to nonspecific nucleotide insertions, dele­tions...allow blocking the PAM sequence (e.g. if it would lead to a missense or nonsense mutation), blocking the...the PAM as possible (see below to find out why). Please check our recent studies for details (Paquet, Kwart...28102837. Additional Resources on the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design ...
Showing: 541 - 560 of 1018 results