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We narrowed to 1,027 results for: LEA

Showing: 541 - 560 of 1027 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...desired recombination event will remove NeoR only and leave exon 2 floxed, as seen in line 4 of Figure 4. Since...tools for creating knockout animals or cell lines, please consider sharing them by depositing with Addgene... with CRISPR through Non-Homologous End Joining Learn How the FLEx Switch Can Be Used to Turn Genes On...Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about CRISPR  ...

  2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...Addgene blog Learn about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko...environmental isolates. For example, Peters and colleagues used Mobile-CRISPRi to knockdown individual genes...-CRISPRi. As a proof of principle, Peters and colleagues constructed a 40-member library targeting 20 ...

  3. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...forming noncovalent dimers. This property of EGFP can lead to significant artifacts. If you're using green ...reportedly monomeric FPs are not actually monomeric, at least in practical terms. The propensity of a particular...FP dimerization coupled with POI dimerization can lead to polymerization of the fusion protein. A dimeric... members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized...

  4. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...you are purifying plasmid DNA from bacteria or cleaning up a PCR product, a common material you are trying...protein contamination. However, this ratio can be misleading. Some reports suggest that the A260/280 ratio...reagents used during nucleic acid purification can leave behind a variety of potential contaminants, some...Remember to discard flow-through solutions and use clean tubes? How about spin times, were these long enough...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    ...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...responses that inactivate viral genomes or otherwise clear the virus from the cell population (Kafri et al....Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...stages of infection for efficient cell lysis and release of adenovirus from infected cells. J Virol, 70(...

  6. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...numerous discoveries have been made using the Cas9 nuclease in the absence of genomic alterations. These studies...sequence via a targeting gRNA. But dCas9 does not cleave the DNA. Much of the research using dCas9 has focused...research conducted in the Hodaka Fujii Laboratory, please also see our interview with Dr. Fujii. References..., et al. "A programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." Science337.6096...

  7. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...finding how-to videos on YouTube long before he learned (somewhat successfully) how to text or use Facebook... communication that you enjoy and that plays at least slightly to your strengths. Otherwise, you’re going...cost or freeware alternatives and how steep of a learning curve it will be to get to the necessary level...balance out cons. Flexible. Can allow people to learn in multiple ways from the same experience.  Can ...

  8. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...research interests include proteomics, machine learning, and visualization for big biology. GPS for the...are critical? One hypothesis: the enhancers! This leads us to a three-step approach for designing an experiment...database. Aneesh's research interests span machine learning, proteomics, and user interfaces for math.     ...Catch Up on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate Your Genome...

  9. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...and controls (see Tips below), and by validating leads with other methods. More common are false negatives...levels of bait and prey proteins - overexpression can lead to false interactions. Lowered expression levels...techniques! Minimizing false negatives: Interactions leading to detectable reporter signals depend on a number...problems with one or more of these parameters can lead to a high number of false negatives in Y2H screens...

  10. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...a cooler. Chances are one of your colleagues has a frisbee at least. Use it! 8. Organize If you’re doing...make an effort to build relationships with their colleagues. It can be easy to get caught up in the minutia...true. If your event requires a lot of set up or cleanup, ask for volunteers to help out. Delegate- it will...

  11. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ..., and they stepped up to apply for a grant which lead to their new career path.  A part of the career ...story for most established grant professionals is learning to succeed in grants through trial and error, ...increased their demand for stronger budgets with clear budget narratives, logic models, established partnerships... Throughout these moves and program changes, I learned to write grants for health, housing, economic development...

  12. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ... Make it clear that you are interested in the specific position you are applying to. Be clean and professional...letter, and if possible, send it to a mentor or colleague for a second look and helpful feedback!  What ...     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network Become A Good...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... Flow data is messy, so learning how to gate takes a lot longer than learning how to read a gating strategy...visualization tool, a quadrant will often be too clean for real-world data. Most plots will have a shape...    Of course, there are many more things to learn about when diving into flow cytometry data. There...

  14. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...Cas13, the "collateral" activity of the enzyme can lead to off targets and result in significant, negative...processing pre-CRISPR RNA into mature CRISPR RNA and cleaving RNA at positions defined by the target:spacer ...limited to small deletions and small insertions, leaving a several kilobase-sized gap in the gene editing...Abudayyeh. Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases bioRxiv 2021. ...

  15. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...researchers recognize their MANY transferable skills learned while working in the lab and offering advice on...think of creativity as a form of innovation, it’s clear that creativity is absolutely a transferable skill...get distracted by an email or sidetracked by a colleague. To innovate, you’ll have to focus. Find yourself...(2019). Embrace your weird face your fears and unleash creativity. Gallery Books. Boyes, A. (2019, November...

  16. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...and ssDNA cleavage by canonical base excision repair enzymes. At the same time Cas9n cleaves the opposing...editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/...and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Research...

  17. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... around, the plasmids are randomly positioned, leading to plasmid loss (Diaz et al., 2015).  Figure...designed small, high copy incompatible plasmids that lead to asymmetrical plasmid loss of the usually large...Addgene blog Read all of our Plasmids 101 blog posts Learn more about the origin of replication Find blog posts... Leah Schwiesow...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... proximity of the fluorophore and subsequently leading to a positive signal. The same effect can be achieved...this approach less sensitive in most applications. Learn About Fluorescent Biosensors Light-up aptamers (...assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound malachite...Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling Protein Activity with...

  20. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...also reversible: once the environmental trigger leaves, gene expression is no longer activated. However...N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the different types of Cas9 activators....3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next...More about Genome-wide Screens with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on ...
Showing: 541 - 560 of 1027 results