How to Design Your gRNA for CRISPR Genome Editing
Type
Blog Post
...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...but can often require difficult decisions. For example, would it be better to use a less-active gRNA that...