We narrowed to 928 results for: Mpl;

...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...but can often require difficult decisions. For example, would it be better to use a less-active gRNA that...

...genetic code of living organisms and its societal implications even before the discovery of genes themselves...repaired by homologous recombination with a repair template.  Each zinc finger recognizes a nucleotide triplet... and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing RNA),...us to always consider the ethics and broader implications of our research.  Want the Genome Editing Trading...

...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...another (e.g. green to red, or cyan to green). For example, mEosFP (named after the goddess of dawn in Greek...trafficking and dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable...

...April because devaluing science has alarming implications for our public policies—many of which impact..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...funding, and teaching experience that it takes to complete your resume, without ever thinking that research...research scientist would be a career that could simply cease to exist. But then November hit and panic ensued...

...,000 plasmids, kits, and viral service items we employ dozens of pieces of energy-intensive lab equipment...certainly not the only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide... new programs, we're always striving to set an example of environmental stewardship.  Additional resources...

...in the biological sciences and techniques that simplify and expedite research. We recognize, however, ...Blog is a great way to get your name out there! Completed blog posts are also great to include on CVs and... blog! To sign up to write for the Addgene blog simply email us. We can’t guarantee that all those who...

...be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce a functional ...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease expression...

.... To start the AAV packaged on request process, simply navigate to your plasmid of interest. If it’s eligible...received, we’ll review for technical and legal compliance, and then send you an email with a link to order...There are no quotes or special processes involved; simply request your plasmid of interest in your desired...

...antibodies, for example through the use of less toxic adjuvants; 2) generating complex antibody chips ... assessment of many protein targets in a single sample; 3) producing recombinant, partially humanized ...

...cultural shifts that can have bigger impacts. For example, if you joined an office where everyone used a ...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...time, your organization will have net 30 days to complete payment for your order.  Still have questions?...

...visualization in vivo using ultrasound (for an example see the figure below). In addition to low costs...Approaches to Presenting the Microbial World’ showcased complex connections and ideas in various visualized approaches...gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon gap using gifs”:...

...needs of employees, and the research environment.   What do you hope Addgene will accomplish in the near...creates a compensation philosophy that helps guide employee compensation and also serves as an objective body...

...technique and be able to talk and own these accomplishments. Employers and academic departments want to be able...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...

...heart and soul of Addgene’s mission), but legal complexities can arise when scientists are sharing their ...facilitating the exchange of not just the physical samples, but also the legal documentation that allows you...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...

... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...ability to survive in certain environments, for example. “We’ve been contacted by investigators all over...

...Thousands of mutations have been implicated in cancer. Yet, the majority of them remain uncharacterized... of mutations they identified included gene amplifications and fusions, missense mutations, and indels...scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied genes...

...should receive the clinical sample to test their phages. Because the sample is limited, only the labs that...targeting organisms closely related to the clinical sample. For treatment, it is best to identify a cocktail...

...the guesswork out of protein purification through simple and efficient multiplexing. pCri plasmids are divided...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...

...academic and industry scientists. This partnership complemented each other's strengths: Ginkgo Bioworks’ infrastructure...distribute such a large deposit. Once the project is complete, Ginkgo will have synthesized approximately 3,... their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox Foundation are...