We narrowed to 1,017 results for: PLE;

...and even allowing multiple proteins to be visualized simultaneously. Complementation systems for examining...For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime... with incompatible substrates can also allow multiplexing with experiments that supply both substrates... Multiple Authors...

...Cis/trans activity, PAM requirement, gRNA, and multiplex ability Experimental guidelines and applications... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ...you need more nitty-gritty details — how can I multiplex this enzyme? What other RNA components are required.... For each enzyme, it will also show you how multiplexed arrays would be designed, if that’s your end ...details to refine your search (trans activity, multiplex ability, gRNA design, etc.). With this feature...

...help my research and my career. Here are three examples of how using social media has helped me or my ... can start using Twitter and provide real-life examples of how they and others have used Twitter professionally...also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what...do you do? — Stephani Page, PhD 👩🏾‍🔬💜 (@ThePurplePage) February 13, 2014 2. "The Scientist's Top... for Twitter pros as well, like tip #4: “Follow people for about a week and then unfollow those who do...

... Methylases involved with these processes (for example Dam and Dcm methylases) are independent from the...conversely, activating enzymes such as DpnI. For example, cleavage by XbaI may be blocked due to methylation...those sites will be methylated. Using the same example plasmid as above (which has the XbaI site blocked...methylation by altering your choice of bacteria. For example, if you must use a restriction site that will be...produce DNA that is unmethylated at those sites. Please keep in mind that dam-/dcm- strains may have an...

...labels, since I often work on multiple projects in a given day. For example, instead of naming a file “output...experiments can be found in each folder. This can be a simple .txt document. Record the name, date, and location...folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...result was, so try not to do this. These are the simple rules that I try to follow in my own research. ...

...of the sample due to the smaller size of RANbodies compared to antibodies. Staining with multiple HRP-RANbodies...used at a time. Samples can be sequentially stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies...process that’s difficult for individual labs to complete. The nanobody based RANbody platform from the ...

...Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling too many fragments...methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature...Remember to quantify the DNA concentration of all the samples by gel or by nanodrop to assemble the  fragments...molar ratio. Assembly of the fragments There are multiple ways you can assemble the different parts of a...plasmid. Many vectors contain sequences that are complementary to standard sequencing primers. If not, design...

...outlined in Table 1.   Of course, this is for the simple insertion of a PCR product into a vector. The time...calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours, so at least...published in. That’s also a great way to get to know people doing interesting and relevant work in your field...That way, you can run one PCR, one gel, etc. for multiple plasmids at once. Over time, this can save you... Multiple Authors...

...is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it will proceed to ...degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is ...that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged by its target sequence. Why...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest... the other Cas family members make for useful implements in the scientist toolbox. If your genome engineering...

...ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving skills...problem.  Step Three: Decide on a strategy and implement it.  Step Four: Assess how effective your strategy...the cause. To test the most likely cause, you implement an experiment designed to fix the problem. Then...modifications, and ultimately decided on the changes to implement. As with any skill, there will always be room ...Know your strengths! Research experience presents ample opportunities to practice problem solving skills...

...ccdB aspect, Gateway takes advantage of the same principle that cells will not propagate while expressing...system contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of...interest.   CcdB resistant E. coli strains complete the system But ccdB alone isn’t the only key to...most appropriate method for your own experiments. Please check out Addgene’s Choosing a Molecular Cloning...protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3):735-45. PubMed...

...conduct research that fails less and helps more people, we need to take sex into account. Gendered Innovations...s Health Initiative Hormone Therapy Trial, for example, found that the study saved lives. Reduced use ...analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery and ...experiments—which can lead to failed research. For example, an international research team from Norway and...studies that draw from a body of research that is incomplete (because sex was not analyzed) may harm humans...

... the xanthine produced via complete demethylation of caffeine. For example, the Barrick lab was able to...ever like to write about a recent plasmid deposit please sign up here.     Listen to the Hot Plasmids episode...living biosensor for measuring caffeine content in samples. The Barrick lab and the UT Austin undergraduate... to grow this strain in minimal media supplemented with various caffeinated beverages, but not with caffeine-free...

... the mutation or replacing the gene. A notable example is the treatment of Severe Combined Immune Deficiency...normally functioning T cell repertoire (1). In this example, it was possible to transduce the T-cells outside...based delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual...already have ways to prevent AAV infection. For example, prevalence of neutralizing antibodies against ...: 14566358.  2. Hirsch, M. L., et al. "Self-complementary AAV mediates gene targeting and enhances endonuclease...

...CRISPR is a simple and versatile tool for genome engineering, but its utility is dependent on its ability...tiny DNA cutting ninjas that deliver CRISPR to multiple types of targets: primary cells, embryos, and ...helps form DNA and retroviral-particle or VLPs complexes. The team found that "all-in-one" Nanoblades facilitated...their CRISPR cargo into the zygote. As a proof-of-principle, the team used Nanoblades to disrupt the tyrosinase...no detectable editing. Additionally, off-target spleen editing was not detected and Nanoblade injection...

...coordination to more complex situations such as learning and memory or emotional states, for example anxiety or ...and cause variability in the testing group. For example, if an experimenter picks up a mouse from the home...kHz, produced from fluorescent light bulbs, for example) that are outside the humans’ audible spectrum.... have preferences for specific cell types. For example, AAV1 and AAV9 serotypes have strong neuronal tropism...

...all essential embryonic genes are individually depleted via RNA interference (RNAi). Scientists monitor...AID system discovered in plants to conditionally deplete proteins of interest in C. elegans (Zhang et al...Schwartz and Jorgensen, 2018).  These are just some examples of worm resources and tools openly shared by the...Hammarlund, Marc, et al. "The CeNGEN Project: The Complete Gene Expression Map of an Entire Nervous System... system enables versatile conditional protein depletion in C. elegans." Development 142.24 (2015): 4374...

...extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase (RT... the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting out with... nicking the newly synthesized strand before heteroduplex resolution, which can lead to indels (Anzalone...edited strand as a template during DNA repair. Test multiple nick sites, starting with sites about 50 bp upstream...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935...

...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Using genetically encoded fluorescent proteins coupled with Fibronectin intrabodies generated with mRNA...speed across these different cell types. In one example, H2B-FT was expressed in murine B cells (BaF3),... the plasmids at Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) ...

...bit of advice I can suggest, is to give yourself plenty of time when writing a fellowship proposal. Yes... you learn new techniques and interact with new people. If you are looking to move to a different university...there is an increasing realization that, if we want people to keep contributing to science, it would be helpful...audience. My lay paragraph typically includes a couple of sentences of introduction to my area, a line...great information on ELISA troubleshooting and multiplex assays.   Additional Resources on the Addgene ...