We narrowed to 660 results for: SIM;

1. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   ...efficiency, the split editors transduced at levels similar to that of a single AAV expressing GFP. The lab...

2. Summer SciComm: Preprints

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   Blog Post

   ...reluctance to share their work too openly too soon or simply a lack of awareness of the benefits of preprints...

3. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

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   Blog Post

   ...National Cancer Institute (NCI) has an internship similar to Susan’s NEI internship. Application information...

4. Visualizing Protein Turnover In Situ

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   ...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...

5. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   ...postdocs at other institutions to see if we can similarly convince them to go ahead with raises. What will...

6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ... that actually contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting ...

7. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   Blog Post

   ...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...

8. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...biotechnology today are things like butanol - very simple molecules - a few carbons and oxygens. Nothing ...

9. Plasmids 101: Cre-lox

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   ...site-directed recombination technology very conceptually similar to Cre-lox, with flippase (Flp) and the short flippase...

10. Working with Nuclear Receptors

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    Blog Post

    ...website, the new version of which will feature a simplified user interface, expanded numbers of curated molecules...

11. Design Tips for Prime Editing

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    Blog Post

    ...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...

12. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    ... of the transcribed ncRNA (Jiang et al. 2014).  Simple knock-out experiments are trickier when it comes...

13. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post

    ...first round of detection are actually gone. First, simply repeat the imaging step to check if any secondary...

14. Hot Plasmids February 2024

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    Blog Post

    ...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...

15. Engaging with science and society at pgEd

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    ...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...

16. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post

    ...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...

17. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ... permit the use of multiple FRET biosensors simultaneously would further increase the amount of correlated...

18. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...these colors so that your text is easily read. Similarly, be cognizant of audience members that are color-blind...

19. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    Blog Post

    ...activity, commonly via light-activated ion channels. Similarly, chemogenetic tools are designed channels that...

20. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...system from Jiazhang Lian’s lab that enables simultaneous gene activation, repression, and editing with...