We narrowed to 718 results for: STO

1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

   Type

   Blog Post

   ... system. TF screens can be designed to identify custom TF programs to acutely initiate disease onset, ...

2. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   ...easily started and easily credited. And nothing stops these one-time favors from becoming starting points...

3. Early Career Researcher Toolbox: Free Tools for Science Writing

   Type

   Blog Post

   ... saved to your Mendeley library. The library is stored in the cloud, so you can access it from anywhere...

4. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

   Type

   Blog Post

   ...persistent and exist online indefinitely. PIDs historically referred to objects that exist online, but this...

5. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   ...publication:         References 1. Richardson, Christopher D., et al. "Enhancing homology-directed genome...

6. Components of CRISPR/Cas9

   Type

   Blog Post

   ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

7. Advice for Scientists Starting a Lab

   Type

   Blog Post

   ...tips for starting a lab. Or share your success stories below. And don't forget to stay tuned for the last...

8. Finding and Joining Your Dream Lab

   Type

   Blog Post

   ... hires are simpler because of their documented history in science. Potential graduate students typically...

9. Plan Your Experiment

   Type

   Guide

   ...using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer...overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service...paired with restriction enzymes for further customization Usually paired with a sequencing method Can’...

10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

11. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    ...mutate in the genome) and an out-of-frame H2B (histone)-GFP reporter into the genome of mammalian cells...

12. Antibodies 101: ChIP

    Type

    Blog Post

    ...antibody on crosslinked proteins. In the case of histone modifying proteins, which bind quite strongly to...

13. Antibodies 101: Isotypes

    Type

    Blog Post

    ...antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western...

14. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    ...transgene. One such marker is the white gene, which restores eye color to fly lines where the endogenous white...

15. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    ...deposited at Addgene was used to produce the glioblastoma model for this study (Ref 6)). In addition, ...

16. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    ...fluorescent proteins. This weak emission hasn’t stopped some scientists from using chemiluminescent proteins...

17. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    ...one assay per gene approach that has been the historical standard. Specifically, in our recent publications...

18. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    ...mechanisms by which retrograde AAV work are not yet understood, one thing is clear: this application opens a...

19. Viral Vectors 101: Viral Applications

    Type

    Blog Post

    ...in your gene of interest, for example. Some customization is required, but many viral constructs have ...

20. Viral Vectors 101: Viruses as Biological Tools

    Type

    Blog Post

    ...associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows...